Ecological fitness impairments induced by chronic exposure to polyvinyl chloride nanospheres in Daphnia magna

The aim of this study was to evaluate the effects of chronic exposure (21 days) to an environmentally relevant concentration (10 μg/L) of two different nanoplastic (NP) polymers on the aquatic model organism Daphnia magna. This study examined the impact of exposure to 200 nm polystyrene nanoplastics (PS-NPs) and polyvinyl chloride nanoplastics (PVC-NPs), which had an average size similar to that of PS-NPs (ranging from 50 nm to 350 nm). The effects of polymer exposure on morphometric parameters, number of molts, swimming behaviour, and reproductive outcomes were evaluated. The findings indicate that PVC exposure induced higher body dimensions, while both polymers resulted in an increase in molting behaviour. Moreover, exposure to PVC-NPs had a negative impact on the reproduction of D. magna, as evidenced by a delay in the day of the first brood, a reduction in the total number of offspring produced, and, consequently, a slower population growth rate. It is hypothesised that the ingestion of PVC-NPs by D. magna may have resulted in an impairment of ecdysone hormone functionality and that the increased moulting events potentially representing an adaptive response to the negative effects of PVC-NP adhesion to the organism's body surfaces. These two organisms' responses could concur to explain the observed effects. This study identified the fitness impairments caused by exposure to PVC-NPs, which can lead to relevant ecological consequences. The comparative analysis of the effects induced by two types of polymers has revealed the generation of disparate hazards to D. magna. Furthermore, the chemical composition appears to be a pivotal factor in the onset of these effects. It can therefore be stated that PS is not a suitable standard for representing the toxicity of all plastics.