BackgroundEscherichia coli cells are the most frequently used hosts in recombinant protein production processes and mainly require molecules such as IPTG or pure lactose as inducers of heterologous expression. A possible way to reduce the production costs is to replace traditional inducers with waste materials such as cheese whey permeate (CWP). CWP is a secondary by-product generated from the production of the valuable whey proteins, which are obtained from ultrafiltration of cheese whey, a main by-product of the dairy industry, which is rich in lactose.ResultsThe effects of CWP collected from an Italian plant were compared with those of traditional inducers on the production of two model proteins (i.e., green fluorescent protein and the toxic Q55 variant of ataxin-3), in E. coli BL21 (DE3) cells. It was found that the high lactose content of CWP (165 g/L) and the antioxidant properties of its micronutrients (vitamins, cofactors and osmolytes) sustain production yields similar to those obtained with traditional inducers, accompanied by the improvement of cell fitness.ConclusionsCWP has proven to be an effective and low-cost alternative inducer to produce recombinant proteins. Its use thus combines the advantage of exploiting a waste product with that of reducing the production costs of recombinant proteins.

de Divitiis, M., Ami, D., Pessina, A., Palmioli, A., Sciandrone, B., Airoldi, C., et al. (2023). Cheese-whey permeate improves the fitness of Escherichia coli cells during recombinant protein production. BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS, 16(1) [10.1186/s13068-023-02281-8].

Cheese-whey permeate improves the fitness of Escherichia coli cells during recombinant protein production

de Divitiis, Marcella
Primo
;
Ami, Diletta;Pessina, Alex;Palmioli, Alessandro;Sciandrone, Barbara;Airoldi, Cristina;Regonesi, Maria Elena;Brambilla, Luca;Lotti, Marina;Natalello, Antonino;Brocca, Stefania
Penultimo
;
Mangiagalli, Marco
Ultimo
2023

Abstract

BackgroundEscherichia coli cells are the most frequently used hosts in recombinant protein production processes and mainly require molecules such as IPTG or pure lactose as inducers of heterologous expression. A possible way to reduce the production costs is to replace traditional inducers with waste materials such as cheese whey permeate (CWP). CWP is a secondary by-product generated from the production of the valuable whey proteins, which are obtained from ultrafiltration of cheese whey, a main by-product of the dairy industry, which is rich in lactose.ResultsThe effects of CWP collected from an Italian plant were compared with those of traditional inducers on the production of two model proteins (i.e., green fluorescent protein and the toxic Q55 variant of ataxin-3), in E. coli BL21 (DE3) cells. It was found that the high lactose content of CWP (165 g/L) and the antioxidant properties of its micronutrients (vitamins, cofactors and osmolytes) sustain production yields similar to those obtained with traditional inducers, accompanied by the improvement of cell fitness.ConclusionsCWP has proven to be an effective and low-cost alternative inducer to produce recombinant proteins. Its use thus combines the advantage of exploiting a waste product with that of reducing the production costs of recombinant proteins.
Articolo in rivista - Articolo scientifico
Dairy industry; Escherichia coli BL21 (DE3); Micro-FTIR; pET expression system; Recombinant proteins; Waste valorization;
English
23-feb-2023
2023
16
1
30
open
de Divitiis, M., Ami, D., Pessina, A., Palmioli, A., Sciandrone, B., Airoldi, C., et al. (2023). Cheese-whey permeate improves the fitness of Escherichia coli cells during recombinant protein production. BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS, 16(1) [10.1186/s13068-023-02281-8].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/410418
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