Biofluid analysis by optical spectroscopy techniques is attracting considerable interest due to its potential to revolutionize diagnostics and precision medicine, particularly for neurodegenerative diseases. However, the lack of effective biomarkers combined with the unaccomplished identification of convenient biofluids has drastically hampered optical advancements in clinical diagnosis and monitoring of neurodegenerative disorders. Here, we show that vibrational spectroscopy applied to human tears opens a new route, offering a non-invasive, label-free identification of a devastating disease such as amyotrophic lateral sclerosis (ALS). Our proposed approach has been validated using two widespread techniques, namely, Fourier transform infrared (FTIR) and Raman microspectroscopies. In conjunction with multivariate analysis, this vibrational approach made it possible to discriminate between tears from ALS patients and healthy controls (HCs) with high specificity (∼97% and ∼100% for FTIR and Raman spectroscopy, respectively) and sensitivity (∼88% and ∼100% for FTIR and Raman spectroscopy, respectively). Additionally, the investigation of tears allowed us to disclose ALS spectroscopic markers related to protein and lipid alterations, as well as to a reduction of the phenylalanine level, in comparison with HCs. Our findings show that vibrational spectroscopy is a new potential ALS diagnostic approach and indicate that tears are a reliable and non-invasive source of ALS biomarkers.

Ami, D., Duse, A., Mereghetti, P., Cozza, F., Ambrosio, F., Ponzini, E., et al. (2021). Tear-Based Vibrational Spectroscopy Applied to Amyotrophic Lateral Sclerosis. ANALYTICAL CHEMISTRY, 93(51), 16995-17002 [10.1021/acs.analchem.1c02546].

Tear-Based Vibrational Spectroscopy Applied to Amyotrophic Lateral Sclerosis

Ami D.;Duse A.;Cozza F.;Ponzini E.;Grandori R.;Tavazzi S.;Pezzoli F.
;
Natalello A.
2021

Abstract

Biofluid analysis by optical spectroscopy techniques is attracting considerable interest due to its potential to revolutionize diagnostics and precision medicine, particularly for neurodegenerative diseases. However, the lack of effective biomarkers combined with the unaccomplished identification of convenient biofluids has drastically hampered optical advancements in clinical diagnosis and monitoring of neurodegenerative disorders. Here, we show that vibrational spectroscopy applied to human tears opens a new route, offering a non-invasive, label-free identification of a devastating disease such as amyotrophic lateral sclerosis (ALS). Our proposed approach has been validated using two widespread techniques, namely, Fourier transform infrared (FTIR) and Raman microspectroscopies. In conjunction with multivariate analysis, this vibrational approach made it possible to discriminate between tears from ALS patients and healthy controls (HCs) with high specificity (∼97% and ∼100% for FTIR and Raman spectroscopy, respectively) and sensitivity (∼88% and ∼100% for FTIR and Raman spectroscopy, respectively). Additionally, the investigation of tears allowed us to disclose ALS spectroscopic markers related to protein and lipid alterations, as well as to a reduction of the phenylalanine level, in comparison with HCs. Our findings show that vibrational spectroscopy is a new potential ALS diagnostic approach and indicate that tears are a reliable and non-invasive source of ALS biomarkers.
Articolo in rivista - Articolo scientifico
Scientifica
spectroscopy, Raman, Fourier transform infrared, amyotrophic lateral sclerosis;
English
Ami, D., Duse, A., Mereghetti, P., Cozza, F., Ambrosio, F., Ponzini, E., et al. (2021). Tear-Based Vibrational Spectroscopy Applied to Amyotrophic Lateral Sclerosis. ANALYTICAL CHEMISTRY, 93(51), 16995-17002 [10.1021/acs.analchem.1c02546].
Ami, D; Duse, A; Mereghetti, P; Cozza, F; Ambrosio, F; Ponzini, E; Grandori, R; Lunetta, C; Tavazzi, S; Pezzoli, F; Natalello, A
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/356770
Citazioni
  • Scopus 2
  • ???jsp.display-item.citation.isi??? 1
Social impact