The detection and quantification of immunoglobulin free light chains in serum and urine is recommended for the diagnosis and monitoring of monoclonal gammopathies according to the guidelines of the International Myeloma Working Group (IMWG). Several tests are currently available in the clinical laboratory to detect and quantify free light chains but although quality, efficiency, and effectiveness have been improved, the results are still variable and poorly harmonized and standardized. The present review article wants to analyze these aspects, with a keen eye on techniques, such as mass spectrometry, that could replace in the practical clinical laboratory the current methods including Bence-Jones protein assay and free light chain immunoassays.

Sarto, C., Intra, J., Fania, C., Brivio, R., Brambilla, P., & Leoni, V. (2021). Monoclonal free light chain detection and quantification: Performances and limits of available laboratory assays. CLINICAL BIOCHEMISTRY, 95(September 2021), 28-33 [10.1016/j.clinbiochem.2021.05.006].

Monoclonal free light chain detection and quantification: Performances and limits of available laboratory assays

Sarto C.
Primo
;
Intra J.
Secondo
;
Fania C.;Brivio R.;Brambilla P.;Leoni V.
Ultimo
2021

Abstract

The detection and quantification of immunoglobulin free light chains in serum and urine is recommended for the diagnosis and monitoring of monoclonal gammopathies according to the guidelines of the International Myeloma Working Group (IMWG). Several tests are currently available in the clinical laboratory to detect and quantify free light chains but although quality, efficiency, and effectiveness have been improved, the results are still variable and poorly harmonized and standardized. The present review article wants to analyze these aspects, with a keen eye on techniques, such as mass spectrometry, that could replace in the practical clinical laboratory the current methods including Bence-Jones protein assay and free light chain immunoassays.
Articolo in rivista - Review Essay
Electrophoresis; Free light chains; Laboratory methods; Mass spectrometry; Monoclonal gammopathy;
English
28
33
6
Sarto, C., Intra, J., Fania, C., Brivio, R., Brambilla, P., & Leoni, V. (2021). Monoclonal free light chain detection and quantification: Performances and limits of available laboratory assays. CLINICAL BIOCHEMISTRY, 95(September 2021), 28-33 [10.1016/j.clinbiochem.2021.05.006].
Sarto, C; Intra, J; Fania, C; Brivio, R; Brambilla, P; Leoni, V
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/319636
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