Microalgae are a precious source of polyunsaturated fatty acids (PUFA), however extraction is difficult due to the peculiar microalgae cell structure. Here we describe a new method based on the application of chitosan nanoaggregates as CO2 responsive emulsifier, used to promote the swelling of algae cell wall and the formation of a large oil – ethanol interphase area during the ethanolysis. Tests were carried out with Pseudokirchneriella subcapitata and Nannochloropsis sp. at different biomass/ethanol/chitosan ratios. CO2 was added to trigger demulsification to promote an easy recovery of the lipid fraction. The highest yields in PUFA were obtained with Nannochloropsis sp. (207.9 mg/g of oil) using 0.4% wt of chitosan and 1:10 biomass:ethanol ratio; 43.6 mg/g of linolenic acid were obtained from Pseudokirchneriella subcapitata. Overall, because the method employs ethanol, a generally recognized as safe (GRAS) solvent, and food grade additives, it is suited for the preparation of PUFA supplements.

Saliu, F., Magoni, C., Torelli, A., Cozza, R., Lasagni, M., Labra, M. (2021). Omega-3 rich oils from microalgae: A chitosan mediated in situ transesterification method. FOOD CHEMISTRY, 337(1 February 2021) [10.1016/j.foodchem.2020.127745].

Omega-3 rich oils from microalgae: A chitosan mediated in situ transesterification method

Saliu, Francesco
;
Magoni, Chiara;Lasagni, Marina;Labra, Massimo
2021

Abstract

Microalgae are a precious source of polyunsaturated fatty acids (PUFA), however extraction is difficult due to the peculiar microalgae cell structure. Here we describe a new method based on the application of chitosan nanoaggregates as CO2 responsive emulsifier, used to promote the swelling of algae cell wall and the formation of a large oil – ethanol interphase area during the ethanolysis. Tests were carried out with Pseudokirchneriella subcapitata and Nannochloropsis sp. at different biomass/ethanol/chitosan ratios. CO2 was added to trigger demulsification to promote an easy recovery of the lipid fraction. The highest yields in PUFA were obtained with Nannochloropsis sp. (207.9 mg/g of oil) using 0.4% wt of chitosan and 1:10 biomass:ethanol ratio; 43.6 mg/g of linolenic acid were obtained from Pseudokirchneriella subcapitata. Overall, because the method employs ethanol, a generally recognized as safe (GRAS) solvent, and food grade additives, it is suited for the preparation of PUFA supplements.
Articolo in rivista - Articolo scientifico
Chitosan; Eicosapentaenoic acid (EPA); Emulsion; Functional food; Lipid; Microalgae; Polyunsaturated fatty acids (PUFA); Supplements;
English
5-ago-2020
2021
337
1 February 2021
127745
none
Saliu, F., Magoni, C., Torelli, A., Cozza, R., Lasagni, M., Labra, M. (2021). Omega-3 rich oils from microalgae: A chitosan mediated in situ transesterification method. FOOD CHEMISTRY, 337(1 February 2021) [10.1016/j.foodchem.2020.127745].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/282114
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