The way pollinators gather resources may play a key role for buffering their population declines. Social pollinators like bumblebees could adjust their foraging after significant workforce reductions to keep provisions to the colony optimal, especially in terms of pollen diversity and quantity. To test what effects a workforce reduction causes on the foraging for pollen, commercially-acquired colonies of the bumblebee Bombus terrestris were allowed to forage in the field and they were experimentally manipulated by removing half the number of workers. For each bumblebee, the pollen pellets were taxonomically identified with DNA metabarcoding of the ITS2 region followed by a statistical filtering based on ROC curves to filter out underrepresented OTUs. Video cameras and network analyses were employed to investigate changes in foraging strategies and behaviour. After filtering out the false-positives, HTS metabarcoding yielded a high plant diversity in the pollen pellets; for plant identity and pollen quantity traits no differences emerged between samples from treated and from control colonies, suggesting that plant choice was influenced mainly by external factors such as the plant phenology. The colonies responded to the removal of 50% of their workers by increasing the foraging activity of the remaining workers, while only negligible changes were found in diet breadth and indices describing the structure of the pollen transport network. Therefore, a consistency in the bumblebees’ feeding strategies emerges in the short term despite the lowered workforce.

Biella, P., Tommasi, N., Akter, A., Guzzetti, L., Klecka, J., Sandionigi, A., et al. (2019). Foraging strategies are maintained despite workforce reduction: A multidisciplinary survey on the pollen collected by a social pollinator. PLOS ONE, 14(11) [10.1371/journal.pone.0224037].

Foraging strategies are maintained despite workforce reduction: A multidisciplinary survey on the pollen collected by a social pollinator

Biella, Paolo
Primo
;
Tommasi, Nicola
Secondo
;
Guzzetti, Lorenzo;Sandionigi, Anna;Labra, Massimo
Penultimo
;
Galimberti, Andrea
Ultimo
2019

Abstract

The way pollinators gather resources may play a key role for buffering their population declines. Social pollinators like bumblebees could adjust their foraging after significant workforce reductions to keep provisions to the colony optimal, especially in terms of pollen diversity and quantity. To test what effects a workforce reduction causes on the foraging for pollen, commercially-acquired colonies of the bumblebee Bombus terrestris were allowed to forage in the field and they were experimentally manipulated by removing half the number of workers. For each bumblebee, the pollen pellets were taxonomically identified with DNA metabarcoding of the ITS2 region followed by a statistical filtering based on ROC curves to filter out underrepresented OTUs. Video cameras and network analyses were employed to investigate changes in foraging strategies and behaviour. After filtering out the false-positives, HTS metabarcoding yielded a high plant diversity in the pollen pellets; for plant identity and pollen quantity traits no differences emerged between samples from treated and from control colonies, suggesting that plant choice was influenced mainly by external factors such as the plant phenology. The colonies responded to the removal of 50% of their workers by increasing the foraging activity of the remaining workers, while only negligible changes were found in diet breadth and indices describing the structure of the pollen transport network. Therefore, a consistency in the bumblebees’ feeding strategies emerges in the short term despite the lowered workforce.
Articolo in rivista - Articolo scientifico
pollinators
English
2019
14
11
e0224037
open
Biella, P., Tommasi, N., Akter, A., Guzzetti, L., Klecka, J., Sandionigi, A., et al. (2019). Foraging strategies are maintained despite workforce reduction: A multidisciplinary survey on the pollen collected by a social pollinator. PLOS ONE, 14(11) [10.1371/journal.pone.0224037].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/260210
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