Background and Aims: It has been established that the inflammatory component represents a significant contribution in the background of clinically recognizable risk factors of atherosclerosis, for example, hypertension, hyperglycemia, dyslipidemia, obesity. The aim of this study was to evaluate systemic and tissue-specific inflammation in pigs fed with high-fat diet. Methods: 21 pigs were kept for 16 weeks on a standard diet (SD; 3.6% lipids; n = 7) or a high-fat diet (HFD; 22% lipids, 5% cholesterol; n = 14). Seven HFD fed pigs received high-dose atorvastatin (80 mg/die) starting after 8 weeks of hypercholesterolemic diet until the end of the experimental procedure. Results: HFD administration significantly raised the number of circulating leukocytes, leading to a “hypercholesterolemia-associated monocytosis” and promoting the synthesis of several pro-inflammatory molecules. Hypercholesterolemia induced adipocytes hypertrophy and T-lymphocytes infiltration in abdominal white adipose tissue (WAT). HFD-fed pigs displayed liver inflammation, hepatic stellate cells activation with a concomitant increased infiltration of macrophages, T- and B-lymphocytes. Inflammation extended beyond liver and WAT with increased macrophage content in lung parenchyma. Atorvastatin abolished WAT inflammation by reducing adipocyte area and the number of infiltrating T-lymphocytes. In the liver, atorvastatin decreased hepatic stellate cells activation and the inflammatory infiltrate and lowered the amount of macrophages in lung parenchima of HFD-fed pigs. Conclusion: Hypercholesterolemia significantly raised the amount of circulating leukocytes and exacerbated the inflammatory response in WAT, liver and lung. Atorvastatin treatment markedly decreased systemic and tissue-specific inflammatory markers by preventing the development of an inflammatory milieu and the accumulation of infiltrating leukocytes

Giovannoni, R., Busnelli, M., Cerrito, M., Froio, A., Manzini, S., Vargiolu, A., et al. (2010). Evaluation of systemic and tissue-specific inflammation as risk factor for atherosclerosis in pigs fed with high-fat diet. In Abstracts of the 78th EAS Congress (pp.46-46). Amsterdam : Elsevier Science [10.1016/S1567-5688(10)70207-5].

Evaluation of systemic and tissue-specific inflammation as risk factor for atherosclerosis in pigs fed with high-fat diet

GIOVANNONI, ROBERTO;BUSNELLI, MARCO;CERRITO, MARIA GRAZIA;FROIO, ALBERTO;MANZINI, STEFANO;VARGIOLU, ALESSIA;LEONE, BIAGIO EUGENIO;BIASI, GIORGIO MARIA;LAVITRANO, MARIALUISA
2010

Abstract

Background and Aims: It has been established that the inflammatory component represents a significant contribution in the background of clinically recognizable risk factors of atherosclerosis, for example, hypertension, hyperglycemia, dyslipidemia, obesity. The aim of this study was to evaluate systemic and tissue-specific inflammation in pigs fed with high-fat diet. Methods: 21 pigs were kept for 16 weeks on a standard diet (SD; 3.6% lipids; n = 7) or a high-fat diet (HFD; 22% lipids, 5% cholesterol; n = 14). Seven HFD fed pigs received high-dose atorvastatin (80 mg/die) starting after 8 weeks of hypercholesterolemic diet until the end of the experimental procedure. Results: HFD administration significantly raised the number of circulating leukocytes, leading to a “hypercholesterolemia-associated monocytosis” and promoting the synthesis of several pro-inflammatory molecules. Hypercholesterolemia induced adipocytes hypertrophy and T-lymphocytes infiltration in abdominal white adipose tissue (WAT). HFD-fed pigs displayed liver inflammation, hepatic stellate cells activation with a concomitant increased infiltration of macrophages, T- and B-lymphocytes. Inflammation extended beyond liver and WAT with increased macrophage content in lung parenchyma. Atorvastatin abolished WAT inflammation by reducing adipocyte area and the number of infiltrating T-lymphocytes. In the liver, atorvastatin decreased hepatic stellate cells activation and the inflammatory infiltrate and lowered the amount of macrophages in lung parenchima of HFD-fed pigs. Conclusion: Hypercholesterolemia significantly raised the amount of circulating leukocytes and exacerbated the inflammatory response in WAT, liver and lung. Atorvastatin treatment markedly decreased systemic and tissue-specific inflammatory markers by preventing the development of an inflammatory milieu and the accumulation of infiltrating leukocytes
No
poster
atherosclerosis; inflammation
English
78th EAS Congress
Giovannoni, R., Busnelli, M., Cerrito, M., Froio, A., Manzini, S., Vargiolu, A., et al. (2010). Evaluation of systemic and tissue-specific inflammation as risk factor for atherosclerosis in pigs fed with high-fat diet. In Abstracts of the 78th EAS Congress (pp.46-46). Amsterdam : Elsevier Science [10.1016/S1567-5688(10)70207-5].
Giovannoni, R; Busnelli, M; Cerrito, M; Froio, A; Manzini, S; Vargiolu, A; Leone, B; Forni, M; Bacci, M; Biasi, G; Lavitrano, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/16525
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