Particulate matter (PM) exposure is related to diverse health effects, ranging from respiratory and cardiovascular diseases, to cancer induction; the determination of the elicited effects can be consequence of PM characteristics. We have previously reported (Longhin et al., 2013) that Milan PM composition is related to the sampling season and the particles size, and these properties are responsible for different in vitro effects. Briefly, PM2.5 is dominated by combustion derived particles, consisting of a carbonaceous core with organic and inorganic compounds adsorbed on its surface. In this fraction, organic components are mostly present in winter season, and as a consequence, winter PM2.5 induces a high level of ROS formation and a DNA damaging potential. PM10 derives from abrasion and resuspension processes, and is enriched in crustal material (metals) and biological components (endotoxins), especially during summer season; thus human cells exposed to summer PM10 have a strong pro-inflammatory response. We have now investigated the global gene and microRNA expression changes by GeneChip technology in human bronchial epithelial cells (BEAS-2B) exposed to single dose of Milan winter PM2.5 or summer PM10 for 24 h PM2.5 and PM10 respectively modulated 162 and 144 genes, with 2 fold values respect to the control. Gene prioritization analysis by Toppgene revealed that PM exposure modulates molecular pathways such as TNF and NFKB signalling. Moreover season of sampling and PM size fraction have a specific impact in the regulation of miRNAs expression associated to inflammation and cancer.
Longhin, E., Capasso, L., Battaglia, C., Cosentino, C., Proverbio, M., Cifola, I., et al. (2014). Gene expression profiling of human bronchial epithelial cells exposed to seasonal Milan particulate matter. Intervento presentato a: 50th Congress of the European Societies of Toxicology (EUROTOX), EDIMBURGO [10.1016/j.toxlet.2014.06.278].
Gene expression profiling of human bronchial epithelial cells exposed to seasonal Milan particulate matter
LONGHIN, ELEONORA MARTA;CAPASSO, LAURA;CAMATINI, MARINA CARLA;GUALTIERI, MAURIZIO
2014
Abstract
Particulate matter (PM) exposure is related to diverse health effects, ranging from respiratory and cardiovascular diseases, to cancer induction; the determination of the elicited effects can be consequence of PM characteristics. We have previously reported (Longhin et al., 2013) that Milan PM composition is related to the sampling season and the particles size, and these properties are responsible for different in vitro effects. Briefly, PM2.5 is dominated by combustion derived particles, consisting of a carbonaceous core with organic and inorganic compounds adsorbed on its surface. In this fraction, organic components are mostly present in winter season, and as a consequence, winter PM2.5 induces a high level of ROS formation and a DNA damaging potential. PM10 derives from abrasion and resuspension processes, and is enriched in crustal material (metals) and biological components (endotoxins), especially during summer season; thus human cells exposed to summer PM10 have a strong pro-inflammatory response. We have now investigated the global gene and microRNA expression changes by GeneChip technology in human bronchial epithelial cells (BEAS-2B) exposed to single dose of Milan winter PM2.5 or summer PM10 for 24 h PM2.5 and PM10 respectively modulated 162 and 144 genes, with 2 fold values respect to the control. Gene prioritization analysis by Toppgene revealed that PM exposure modulates molecular pathways such as TNF and NFKB signalling. Moreover season of sampling and PM size fraction have a specific impact in the regulation of miRNAs expression associated to inflammation and cancer.
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