Serotonin (5-HT) and the serotonergic system have recently been indicated as modulators of adult hippocampal neurogenesis. In this study, we evaluated the role of 5-HT on the functional features in neurospheres derived from adult neural stem cells (ANSC). We cultured neurospheres derived from mouse hippocampus in serum-free medium containing epidermal (EGF) and type-2 fibroblast growth factor (FGF2). Under these conditions ANSC expressed both isoforms of tryptophane-hydroxylase (TPH) and produced 5-HT. Blocking TPH function by para-chlorophenylalanine (PCPA) reduced ANSC proliferation, which was rescued by exogenous 5-HT. 5-HT action on ANSC was mediated predominantly by the serotonin receptor subtype 5-HT1A and, to a lesser extent, through the 5-HT2C (receptor) subtype, as shown by selectively antagonizing these receptors. Finally, we documented a 5-HT-induced increase of ANSC migration activity. In summary, we demonstrated a powerful serotonergic impact on ANSC functional features, which was mainly mediated by 5-HT1A receptors.

Benninghoff, J., Gritti, A., Rizzi, M., Lamorte, G., Schloesser, R., Schmitt, A., et al. (2010). Serotonin depletion hampers survival and proliferation in neurospheres derived from adult neural stem cells. NEUROPSYCHOPHARMACOLOGY, 35(4), 893-903 [10.1038/npp.2009.181].

Serotonin depletion hampers survival and proliferation in neurospheres derived from adult neural stem cells

VESCOVI, ANGELO LUIGI
2010

Abstract

Serotonin (5-HT) and the serotonergic system have recently been indicated as modulators of adult hippocampal neurogenesis. In this study, we evaluated the role of 5-HT on the functional features in neurospheres derived from adult neural stem cells (ANSC). We cultured neurospheres derived from mouse hippocampus in serum-free medium containing epidermal (EGF) and type-2 fibroblast growth factor (FGF2). Under these conditions ANSC expressed both isoforms of tryptophane-hydroxylase (TPH) and produced 5-HT. Blocking TPH function by para-chlorophenylalanine (PCPA) reduced ANSC proliferation, which was rescued by exogenous 5-HT. 5-HT action on ANSC was mediated predominantly by the serotonin receptor subtype 5-HT1A and, to a lesser extent, through the 5-HT2C (receptor) subtype, as shown by selectively antagonizing these receptors. Finally, we documented a 5-HT-induced increase of ANSC migration activity. In summary, we demonstrated a powerful serotonergic impact on ANSC functional features, which was mainly mediated by 5-HT1A receptors.
Articolo in rivista - Articolo scientifico
Brain; Hippocampus; Neural progenitors; Neurogenesis; Serotonin; TPH;
English
893
903
11
Benninghoff, J., Gritti, A., Rizzi, M., Lamorte, G., Schloesser, R., Schmitt, A., et al. (2010). Serotonin depletion hampers survival and proliferation in neurospheres derived from adult neural stem cells. NEUROPSYCHOPHARMACOLOGY, 35(4), 893-903 [10.1038/npp.2009.181].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/49312
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