BACKGROUND: Acid aspiration is a complication of general anesthesia. Most animal models developed to define its pathophysiology have focused on the acute (< or =24 h) phase of the injury. The authors describe a model of acid aspiration allowing the study of this type of lung injury over time. METHODS: The authors instilled hydrochloric acid (0.1 m, 1.5 ml/kg) or normal saline in the right bronchus of mice. Lung injury was evaluated at 6 h, 12 h, 24 h, and 2 weeks by assessing arterial blood gases, respiratory system compliance, lung wet weight normalized by body weight, lung myeloperoxidase activity, and histology. Twelve hours and 2 weeks after injury, a computed tomography scan was obtained. RESULTS: In the hydrochloric acid group, arterial oxygen tension decreased (P < 0.05) at 12 and 24 h, whereas it recovered at 2 weeks; respiratory system compliance was lower both at 24 h and 2 weeks (P < 0.05). Lung weight increased at 12 and 24 h (P < 0.05). Myeloperoxidase activity peaked between 6 and 12 h. Computed tomography at 12 h showed that almost 30% of the injured lung was abnormally aerated. Although reduced, the abnormalities were still present at 2 weeks as confirmed by a fibrotic scar well evident at histologic examination. CONCLUSION: The authors characterized a murine model of regional acid aspiration allowing long-term survival. Despite a partial recovery, at 2 weeks the injury persisted, with evidence of fibrosis and lung compliance reduction. This long-term, low-mortality model seems suitable for assessment of the effects of different therapies on lung injury and repair.

Amigoni, M., Bellani, G., Scanziani, M., Masson, S., Bertoli, E., Radaelli, E., et al. (2008). Lung injury and recovery in a murine model of unilateral acid aspiration: functional, biochemical, and morphologic characterization. ANESTHESIOLOGY, 108(6), 1037-1046 [10.1097/ALN.0b013e318173f64f].

Lung injury and recovery in a murine model of unilateral acid aspiration: functional, biochemical, and morphologic characterization

BELLANI, GIACOMO;PATRONITI, NICOLO' ANTONINO;PESENTI, ANTONIO MARIA;
2008-06

Abstract

BACKGROUND: Acid aspiration is a complication of general anesthesia. Most animal models developed to define its pathophysiology have focused on the acute (< or =24 h) phase of the injury. The authors describe a model of acid aspiration allowing the study of this type of lung injury over time. METHODS: The authors instilled hydrochloric acid (0.1 m, 1.5 ml/kg) or normal saline in the right bronchus of mice. Lung injury was evaluated at 6 h, 12 h, 24 h, and 2 weeks by assessing arterial blood gases, respiratory system compliance, lung wet weight normalized by body weight, lung myeloperoxidase activity, and histology. Twelve hours and 2 weeks after injury, a computed tomography scan was obtained. RESULTS: In the hydrochloric acid group, arterial oxygen tension decreased (P < 0.05) at 12 and 24 h, whereas it recovered at 2 weeks; respiratory system compliance was lower both at 24 h and 2 weeks (P < 0.05). Lung weight increased at 12 and 24 h (P < 0.05). Myeloperoxidase activity peaked between 6 and 12 h. Computed tomography at 12 h showed that almost 30% of the injured lung was abnormally aerated. Although reduced, the abnormalities were still present at 2 weeks as confirmed by a fibrotic scar well evident at histologic examination. CONCLUSION: The authors characterized a murine model of regional acid aspiration allowing long-term survival. Despite a partial recovery, at 2 weeks the injury persisted, with evidence of fibrosis and lung compliance reduction. This long-term, low-mortality model seems suitable for assessment of the effects of different therapies on lung injury and repair.
Articolo in rivista - Articolo scientifico
Scientifica
Acid aspiration pneumonia; lung fibrosis, mechanical ventilation, mice
English
1037
1046
Amigoni, M., Bellani, G., Scanziani, M., Masson, S., Bertoli, E., Radaelli, E., et al. (2008). Lung injury and recovery in a murine model of unilateral acid aspiration: functional, biochemical, and morphologic characterization. ANESTHESIOLOGY, 108(6), 1037-1046 [10.1097/ALN.0b013e318173f64f].
Amigoni, M; Bellani, G; Scanziani, M; Masson, S; Bertoli, E; Radaelli, E; Patroniti, N; Di Lelio, A; Pesenti, A; Latini, R
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/4728
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