The regulation of miRNAs is critical to the definition of cell identity and behavior in normal physiology and disease. To date, the dynamics of miRNA degradation and the mechanisms involved in remain largely obscure, in particular, in higher organisms. Here, we developed a pulse-chase approach based on metabolic RNA labeling to calculate miRNA decay rates at genome-wide scale in mammalian cells. Our analysis revealed heterogeneous miRNA half-lives, with many species behaving as stable molecules (T1/2 > 24 h), while others, including passenger miRNAs and a number (25/129) of guide miRNAs, are quickly turned over (T1/2 = 4-14 h). Decay rates were coupled with other features, including genomic organization, transcription rates, structural heterogeneity (isomiRs), and target abundance, measured through quantitative experimental approaches. This comprehensive analysis highlighted functional mechanisms that mediate miRNA degradation, as well as the importance of decay dynamics in the regulation of the miRNA pool under both steady-state conditions and during cell transitions.

Marzi, M., Ghini, F., Cerruti, B., de Pretis, S., Bonetti, P., Giacomelli, C., et al. (2016). Degradation dynamics of microRNAs revealed by a novel pulse-chase approach. GENOME RESEARCH, 26(4), 554-565 [10.1101/gr.198788.115].

Degradation dynamics of microRNAs revealed by a novel pulse-chase approach

Pelizzola M;
2016

Abstract

The regulation of miRNAs is critical to the definition of cell identity and behavior in normal physiology and disease. To date, the dynamics of miRNA degradation and the mechanisms involved in remain largely obscure, in particular, in higher organisms. Here, we developed a pulse-chase approach based on metabolic RNA labeling to calculate miRNA decay rates at genome-wide scale in mammalian cells. Our analysis revealed heterogeneous miRNA half-lives, with many species behaving as stable molecules (T1/2 > 24 h), while others, including passenger miRNAs and a number (25/129) of guide miRNAs, are quickly turned over (T1/2 = 4-14 h). Decay rates were coupled with other features, including genomic organization, transcription rates, structural heterogeneity (isomiRs), and target abundance, measured through quantitative experimental approaches. This comprehensive analysis highlighted functional mechanisms that mediate miRNA degradation, as well as the importance of decay dynamics in the regulation of the miRNA pool under both steady-state conditions and during cell transitions.
Articolo in rivista - Articolo scientifico
miRNA, non coding transcription, RNA degradation, RNA decay, RNA dynamics
English
2016
26
4
554
565
open
Marzi, M., Ghini, F., Cerruti, B., de Pretis, S., Bonetti, P., Giacomelli, C., et al. (2016). Degradation dynamics of microRNAs revealed by a novel pulse-chase approach. GENOME RESEARCH, 26(4), 554-565 [10.1101/gr.198788.115].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/447281
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