N6-methyladenosine (m6A) is the most abundant RNA modification. It has been involved in the regulation of RNA metabolism, including degradation and translation, in both physiological and disease conditions. A recent study showed that m6A-mediated degradation of key transcripts also plays a role in the control of T cells homeostasis and IL-7 induced differentiation. We re-analyzed the omics data from that study and, through the integrative analysis of total and nascent RNA-seq data, we were able to comprehensively quantify T cells RNA dynamics and how these are affected by m6A depletion. In addition to the expected impact on RNA degradation, we revealed a broader effect of m6A on RNA dynamics, which included the alteration of RNA synthesis and processing. Altogether, the combined action of m6A on all major steps of the RNA life-cycle closely re-capitulated the observed changes in the abundance of premature and mature RNA species. Ultimately, our re-analysis extended the findings of the initial study, focused on RNA stability, and proposed a yet unappreciated role for m6A in RNA synthesis and processing dynamics.

Furlan, M., Galeota, E., de Pretis, S., Caselle, M., Pelizzola, M. (2019). M6A-dependent RNA dynamics in T cell differentiation. GENES, 10(1) [10.3390/genes10010028].

M6A-dependent RNA dynamics in T cell differentiation

Pelizzola, M
Ultimo
2019

Abstract

N6-methyladenosine (m6A) is the most abundant RNA modification. It has been involved in the regulation of RNA metabolism, including degradation and translation, in both physiological and disease conditions. A recent study showed that m6A-mediated degradation of key transcripts also plays a role in the control of T cells homeostasis and IL-7 induced differentiation. We re-analyzed the omics data from that study and, through the integrative analysis of total and nascent RNA-seq data, we were able to comprehensively quantify T cells RNA dynamics and how these are affected by m6A depletion. In addition to the expected impact on RNA degradation, we revealed a broader effect of m6A on RNA dynamics, which included the alteration of RNA synthesis and processing. Altogether, the combined action of m6A on all major steps of the RNA life-cycle closely re-capitulated the observed changes in the abundance of premature and mature RNA species. Ultimately, our re-analysis extended the findings of the initial study, focused on RNA stability, and proposed a yet unappreciated role for m6A in RNA synthesis and processing dynamics.
Articolo in rivista - Articolo scientifico
M6A; Mathematical modeling; RNA dynamics; RNA metabolic labeling; RNA modifications; RNA-seq;
English
2019
10
1
28
open
Furlan, M., Galeota, E., de Pretis, S., Caselle, M., Pelizzola, M. (2019). M6A-dependent RNA dynamics in T cell differentiation. GENES, 10(1) [10.3390/genes10010028].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/446758
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