The transgenic technologies represent potent biotechnological tools that allow the generation of genetically modified animals useful for basic research and for biomedical, veterinary, and agricultural applications. Among transgenic techniques, we describe here the sperm-mediated gene transfer methods that is gene transfer based on the spontaneous ability of sperm cells to bind and internalize exogenous DNA and to carry it to oocyte during fertilization, producing genetically modified animals with high efficiency.
Lavitrano, M., Giovannoni, R., Cerrito, M. (2013). Methods for sperm-mediated gene transfer. In D.T. Carrell, K.I. Aston (a cura di), Spermiogenesis and Spermatogenesis: Methods and Protocols (pp. 519-529). Springer [10.1007/978-1-62703-38-0_44].
Methods for sperm-mediated gene transfer
LAVITRANO, MARIALUISA;GIOVANNONI, ROBERTO;CERRITO, MARIA GRAZIA
2013
Abstract
The transgenic technologies represent potent biotechnological tools that allow the generation of genetically modified animals useful for basic research and for biomedical, veterinary, and agricultural applications. Among transgenic techniques, we describe here the sperm-mediated gene transfer methods that is gene transfer based on the spontaneous ability of sperm cells to bind and internalize exogenous DNA and to carry it to oocyte during fertilization, producing genetically modified animals with high efficiency.
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