Introduction of the Lactobacillus casei lactate dehydrogenase (LDH) gene into Saccharomyces cerevisiae under the control of the TPII promoter yielded high LDH levels in batch and chemostat cultures. LDH expression did not affect the dilution rate above which respiro-fermentative metabolism occurred (D-c.) in aerobic, glucose-limited chemostats. Above D-c, the LDH-expressing strain produced both ethanol and lactate, but its overall fermentation rate was the same as in wild-type cultures. Exposure of respiring, LDH-expressing cultures to glucose excess triggered simultaneous ethanol and lactate production. However, the specific glucose consumption rate was not affected, indicating that NADH reoxidation does not control glycolytic flux under these conditions

Brambilla, L., Boldani, D., Compagno, C., Carrera, V., Van Dijken, J., Pronk, J., et al. (1999). NADH reoxidation does not control glycolytic flux during exposure of respiring S. cerevisiae cultures to glucose excess. FEMS MICROBIOLOGY LETTERS, 171(2), 133-140 [10.1016/S0378-1097(98)00589-8].

NADH reoxidation does not control glycolytic flux during exposure of respiring S. cerevisiae cultures to glucose excess

BRAMBILLA, LUCA GIUSEPPE;ALBERGHINA, LILIA;PORRO, DANILO
1999

Abstract

Introduction of the Lactobacillus casei lactate dehydrogenase (LDH) gene into Saccharomyces cerevisiae under the control of the TPII promoter yielded high LDH levels in batch and chemostat cultures. LDH expression did not affect the dilution rate above which respiro-fermentative metabolism occurred (D-c.) in aerobic, glucose-limited chemostats. Above D-c, the LDH-expressing strain produced both ethanol and lactate, but its overall fermentation rate was the same as in wild-type cultures. Exposure of respiring, LDH-expressing cultures to glucose excess triggered simultaneous ethanol and lactate production. However, the specific glucose consumption rate was not affected, indicating that NADH reoxidation does not control glycolytic flux under these conditions
Si
Articolo in rivista - Articolo scientifico
Scientifica
NADH, glucose
English
133
140
8
Brambilla, L., Boldani, D., Compagno, C., Carrera, V., Van Dijken, J., Pronk, J., et al. (1999). NADH reoxidation does not control glycolytic flux during exposure of respiring S. cerevisiae cultures to glucose excess. FEMS MICROBIOLOGY LETTERS, 171(2), 133-140 [10.1016/S0378-1097(98)00589-8].
Brambilla, L; Boldani, D; Compagno, C; Carrera, V; Van Dijken, J; Pronk, J; Ranzi, B; Alberghina, L; Porro, D
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/3552
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