Introduction of the Lactobacillus casei lactate dehydrogenase (LDH) gene into Saccharomyces cerevisiae under the control of the TPII promoter yielded high LDH levels in batch and chemostat cultures. LDH expression did not affect the dilution rate above which respiro-fermentative metabolism occurred (D-c.) in aerobic, glucose-limited chemostats. Above D-c, the LDH-expressing strain produced both ethanol and lactate, but its overall fermentation rate was the same as in wild-type cultures. Exposure of respiring, LDH-expressing cultures to glucose excess triggered simultaneous ethanol and lactate production. However, the specific glucose consumption rate was not affected, indicating that NADH reoxidation does not control glycolytic flux under these conditions
Brambilla, L., Boldani, D., Compagno, C., Carrera, V., Van Dijken, J., Pronk, J., et al. (1999). NADH reoxidation does not control glycolytic flux during exposure of respiring S. cerevisiae cultures to glucose excess. FEMS MICROBIOLOGY LETTERS, 171(2), 133-140 [10.1016/S0378-1097(98)00589-8].
Citazione: | Brambilla, L., Boldani, D., Compagno, C., Carrera, V., Van Dijken, J., Pronk, J., et al. (1999). NADH reoxidation does not control glycolytic flux during exposure of respiring S. cerevisiae cultures to glucose excess. FEMS MICROBIOLOGY LETTERS, 171(2), 133-140 [10.1016/S0378-1097(98)00589-8]. | |
Tipo: | Articolo in rivista - Articolo scientifico | |
Carattere della pubblicazione: | Scientifica | |
Presenza di un coautore afferente ad Istituzioni straniere: | Si | |
Titolo: | NADH reoxidation does not control glycolytic flux during exposure of respiring S. cerevisiae cultures to glucose excess | |
Autori: | Brambilla, L; Boldani, D; Compagno, C; Carrera, V; Van Dijken, J; Pronk, J; Ranzi, B; Alberghina, L; Porro, D | |
Autori: | ||
Data di pubblicazione: | 1999 | |
Lingua: | English | |
Rivista: | FEMS MICROBIOLOGY LETTERS | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/S0378-1097(98)00589-8 | |
Appare nelle tipologie: | 01 - Articolo su rivista |