The Candida rugosa lipase I gene has been expressed in Saccharomyces cerevisiae. The recombinant lipase was efficiently synthesized only following the replacement of the enzyme endogenous leader sequence with the signal peptide of the Kluyveromyces lactis killer toxin. Amount of accumulated lipase was about 10-20 mg/l in batch culture and over 1g/l in a computer-controlled fed-batch fermentation system

Fusetti, F., Brocca, S., Porro, D., & Lotti, M. (1996). Effect of the leader sequence on the expression of recombinant C-rugosa lipase by S-cerevisiae cells. BIOTECHNOLOGY LETTERS, 18(3), 281-286.

Effect of the leader sequence on the expression of recombinant C-rugosa lipase by S-cerevisiae cells

BROCCA, STEFANIA;PORRO, DANILO;LOTTI, MARINA
1996

Abstract

The Candida rugosa lipase I gene has been expressed in Saccharomyces cerevisiae. The recombinant lipase was efficiently synthesized only following the replacement of the enzyme endogenous leader sequence with the signal peptide of the Kluyveromyces lactis killer toxin. Amount of accumulated lipase was about 10-20 mg/l in batch culture and over 1g/l in a computer-controlled fed-batch fermentation system
Articolo in rivista - Articolo scientifico
yeast, lipase, C-rugosa
English
281
286
6
Fusetti, F., Brocca, S., Porro, D., & Lotti, M. (1996). Effect of the leader sequence on the expression of recombinant C-rugosa lipase by S-cerevisiae cells. BIOTECHNOLOGY LETTERS, 18(3), 281-286.
Fusetti, F; Brocca, S; Porro, D; Lotti, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/33310
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