The role of mild oxidative stresses elicited by diethylmaleate (DEM)-induced glutathione depletion in the progression of the yeast cell cycle has been investigated. We found that different wild-type strains are sensitive to oxidative stresses induced by similar DEM doses: approximate to 1 mM on YPD plates, 5-10 mM in shaken flasks, At lower doses, DEM caused a transient decrease in growth rate, largely because of a decreased G(1)-to-S transition. Treatment with higher DEM doses leads to complete growth arrest, with most cells found in the unbudded G(1) phase of the cell cycle. DEM treatment resulted in transcriptional induction of stress-responsive Element (STRE)-controlled genes and was relieved by treatment with the antioxidant N-acetyl cysteine, Reciprocal shift experiments with cdc25 and cdc28 mutants showed that the major cell cycle arrest point was located in the Start area, at or near the CDC25-mediated step, before the step mediated by the CDC28 cyclin-dependent kinase, The DEM-induced G(1) arrest requires a properly regulated RAS pathway and can be bypassed by overexpressing the G(1)-specific cyclin CLN2. However, cells with either a deregulated RAS pathway or overexpressing CLN2 failed to grow and arrested as budded cells, indicating that a second DEM-sensitive cell cycle step exists

Wanke, V., Accorsi, K., Porro, D., Esposito, F., Russo, T., Vanoni, M. (1999). In budding yeast, reactive oxygen species induce both RAS-dependent and RAS-independent cell cycle-specific arrest. MOLECULAR MICROBIOLOGY, 32(4), 753-764 [10.1046/j.1365-2958.1999.01391.x].

In budding yeast, reactive oxygen species induce both RAS-dependent and RAS-independent cell cycle-specific arrest

PORRO, DANILO;Vanoni, ME
1999

Abstract

The role of mild oxidative stresses elicited by diethylmaleate (DEM)-induced glutathione depletion in the progression of the yeast cell cycle has been investigated. We found that different wild-type strains are sensitive to oxidative stresses induced by similar DEM doses: approximate to 1 mM on YPD plates, 5-10 mM in shaken flasks, At lower doses, DEM caused a transient decrease in growth rate, largely because of a decreased G(1)-to-S transition. Treatment with higher DEM doses leads to complete growth arrest, with most cells found in the unbudded G(1) phase of the cell cycle. DEM treatment resulted in transcriptional induction of stress-responsive Element (STRE)-controlled genes and was relieved by treatment with the antioxidant N-acetyl cysteine, Reciprocal shift experiments with cdc25 and cdc28 mutants showed that the major cell cycle arrest point was located in the Start area, at or near the CDC25-mediated step, before the step mediated by the CDC28 cyclin-dependent kinase, The DEM-induced G(1) arrest requires a properly regulated RAS pathway and can be bypassed by overexpressing the G(1)-specific cyclin CLN2. However, cells with either a deregulated RAS pathway or overexpressing CLN2 failed to grow and arrested as budded cells, indicating that a second DEM-sensitive cell cycle step exists
Articolo in rivista - Articolo scientifico
budding yeast, cell cycle
English
1999
32
4
753
764
none
Wanke, V., Accorsi, K., Porro, D., Esposito, F., Russo, T., Vanoni, M. (1999). In budding yeast, reactive oxygen species induce both RAS-dependent and RAS-independent cell cycle-specific arrest. MOLECULAR MICROBIOLOGY, 32(4), 753-764 [10.1046/j.1365-2958.1999.01391.x].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/33304
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