Saccharomyces cerevisiae cells transformed with a multicopy expression vector bearing an in frame STA2-LacZ gene fusion under the control of the galactose inducible UAS(GAL)/CYC1 promoter, secrete beta-galactosidase in the periplasmic space. Fermentation studies showed that with this transformed strain it is possible to reach on lactose high cell densities with both high productivity and high growth yield by means of fed-batch fermentations
Porro, D., Martegani, E., Ranzi, B., Alberghina, L. (1992). Development of high cell-density cultures of engineered saccharomyces-cerevisiae cells able to grow on lactose. BIOTECHNOLOGY LETTERS, 14(11), 1085-1088 [10.1007/BF01021064].
Development of high cell-density cultures of engineered saccharomyces-cerevisiae cells able to grow on lactose
Porro, D;Martegani, E;Alberghina, L.
1992
Abstract
Saccharomyces cerevisiae cells transformed with a multicopy expression vector bearing an in frame STA2-LacZ gene fusion under the control of the galactose inducible UAS(GAL)/CYC1 promoter, secrete beta-galactosidase in the periplasmic space. Fermentation studies showed that with this transformed strain it is possible to reach on lactose high cell densities with both high productivity and high growth yield by means of fed-batch fermentations
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