We report the effects of a strong overexpression of the GAL4 activator protein on the expression of UAS(GAL) regulated genes, obtained by cloning the GAL4 gene and the GAL1-10 upstream activating sequence (UAS(GAL))-lacZ fusion in the same high copy number plasmid. Comparable amounts of active enzyme were obtained by host strains usually producing different levels of cloned proteins due to their different genetic background. The transformed cells constitutively produced low levels of beta-galactosidase (1-2% of total proteins) both in glucose and in raffinose minimal media. Nevertheless, expression was still inducible and a tenfold induction could be rapidly obtained by the addition of 0.5% (w/v) galactose to the culture, even when glucose was still present in the medium

Porro, D., Lotti, M., Martegani, E., Ranzi, B., Alberghina, L. (1992). ENHANCED EXPRESSION OF HETEROLOGOUS PROTEINS BY THE USE OF A SUPERINDUCIBLE VECTOR IN BUDDING YEAST. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 36(5), 655-658.

ENHANCED EXPRESSION OF HETEROLOGOUS PROTEINS BY THE USE OF A SUPERINDUCIBLE VECTOR IN BUDDING YEAST

PORRO, DANILO;LOTTI, MARINA;MARTEGANI, ENZO;ALBERGHINA, LILIA
1992

Abstract

We report the effects of a strong overexpression of the GAL4 activator protein on the expression of UAS(GAL) regulated genes, obtained by cloning the GAL4 gene and the GAL1-10 upstream activating sequence (UAS(GAL))-lacZ fusion in the same high copy number plasmid. Comparable amounts of active enzyme were obtained by host strains usually producing different levels of cloned proteins due to their different genetic background. The transformed cells constitutively produced low levels of beta-galactosidase (1-2% of total proteins) both in glucose and in raffinose minimal media. Nevertheless, expression was still inducible and a tenfold induction could be rapidly obtained by the addition of 0.5% (w/v) galactose to the culture, even when glucose was still present in the medium
Articolo in rivista - Articolo scientifico
yeast, heterologous proteins
English
655
658
4
Porro, D., Lotti, M., Martegani, E., Ranzi, B., Alberghina, L. (1992). ENHANCED EXPRESSION OF HETEROLOGOUS PROTEINS BY THE USE OF A SUPERINDUCIBLE VECTOR IN BUDDING YEAST. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 36(5), 655-658.
Porro, D; Lotti, M; Martegani, E; Ranzi, B; Alberghina, L
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/33288
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