High levels of expression of heterologous proteins (from 5 to 15% of total cell proteins) in the budding yeast Saccharomyces cerevisiae have been obtained previously by the use of the inducible strong hybrid promoter UASGAL/CYC1, in batch as well in continuous cultures. However, in order to maximize the yield of heterologous proteins, a computer controlled fed‐batch fermentation is essential. For this reason we have developed a fed‐batch system based on a semiconductor gas detector that measures ethanol in the outflow gases. The optimal conditions are described for very high production (up to 1550 mg/liter), with both high productivity (up to 100–120 mg/liter/h) and high yield (up to 15 mg of protein/g of dry biomass), of heterologous protein driven by the UASGAL/CYC1 promoter in a completely computer controlled fed‐batch fermentation of budding yeast. However, high production was dependent upon the addition of a large amount of galactose. The process was improved by developing a new, more easily inducible, vector system obtained by subcloning the GAL4 gene
ALBERGHINA, L., PORRO, D., MARTEGANI, E., & RANZI, B. (1991). Efficient production of recombinant DNA proteins in Saccharomyces cerevisiae by controlled high‐cell‐density fermentation. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 14(1), 82-92.
Citazione: | ALBERGHINA, L., PORRO, D., MARTEGANI, E., & RANZI, B. (1991). Efficient production of recombinant DNA proteins in Saccharomyces cerevisiae by controlled high‐cell‐density fermentation. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 14(1), 82-92. | |
Tipo: | Articolo in rivista - Articolo scientifico | |
Carattere della pubblicazione: | Scientifica | |
Presenza di un coautore afferente ad Istituzioni straniere: | No | |
Titolo: | Efficient production of recombinant DNA proteins in Saccharomyces cerevisiae by controlled high‐cell‐density fermentation | |
Autori: | ALBERGHINA, L; PORRO, D; MARTEGANI, E; RANZI, B | |
Autori: | ||
Data di pubblicazione: | 1991 | |
Lingua: | English | |
Rivista: | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1111/j.1470-8744.1991.tb00168.x | |
Appare nelle tipologie: | 01 - Articolo su rivista |