Cell size distributions, obtained either as protein distribution by flow cytometry or as cell volume distribution by a Coulter counter, give relevant information about the growth conditions of populations of budding yeast Saccharomyces cerevisiae. We have previously found a good correlation between these distributions and the growth rate in continuous cultures (Ranzi et al., Biotechnol. Bioeng. 1986, 28, 185–190). We now present determinations of the protein distributions and cell volume distributions during different fed‐batch fermentations performed with a simple on/off controller. Since during the fed‐batch fermentation a true steady state is not obtained, the distributions continuously change with time, but nevertheless we observed a good correlation between the average of both distributions and the actual growth rate. The behavior of the cell size distributions can be interpreted on the basis of a two‐threshold cell cycle model in which both the critical protein content at budding (Ps) and the critical protein content for cell division (Pm) are differently modulated by the growth rate. Additional findings will be presented showing that this model can be used to successfully explain the insurgence and the maintenance of oscillatory states in continuous cultures. Copyright

Alberghina, L., Ranzi, B., Porro, D., Martegani, E. (1991). Flow-cytometry and cell-cycle kinetics in continuous and fed-batch fermentations of budding yeast. BIOTECHNOLOGY PROGRESS, 7(4), 299-304 [10.1021/bp00010a002].

Flow-cytometry and cell-cycle kinetics in continuous and fed-batch fermentations of budding yeast

Alberghina, L;Porro, D;Martegani, E.
1991

Abstract

Cell size distributions, obtained either as protein distribution by flow cytometry or as cell volume distribution by a Coulter counter, give relevant information about the growth conditions of populations of budding yeast Saccharomyces cerevisiae. We have previously found a good correlation between these distributions and the growth rate in continuous cultures (Ranzi et al., Biotechnol. Bioeng. 1986, 28, 185–190). We now present determinations of the protein distributions and cell volume distributions during different fed‐batch fermentations performed with a simple on/off controller. Since during the fed‐batch fermentation a true steady state is not obtained, the distributions continuously change with time, but nevertheless we observed a good correlation between the average of both distributions and the actual growth rate. The behavior of the cell size distributions can be interpreted on the basis of a two‐threshold cell cycle model in which both the critical protein content at budding (Ps) and the critical protein content for cell division (Pm) are differently modulated by the growth rate. Additional findings will be presented showing that this model can be used to successfully explain the insurgence and the maintenance of oscillatory states in continuous cultures. Copyright
Articolo in rivista - Articolo scientifico
Flow-Cytometry,Budding Yeast, Fed-Batch Fermentations
English
299
304
6
Alberghina, L., Ranzi, B., Porro, D., Martegani, E. (1991). Flow-cytometry and cell-cycle kinetics in continuous and fed-batch fermentations of budding yeast. BIOTECHNOLOGY PROGRESS, 7(4), 299-304 [10.1021/bp00010a002].
Alberghina, L; Ranzi, B; Porro, D; Martegani, E
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/33283
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