The complete utilization of raffinose and melibiose by Saccharomyces cerevisiae requires the synthesis and secretion of an α-galactosidase encoded by a single MEL1 gene. The influence of culture conditions on the productivity of α-galactosidase in batch growth of yeast cells has been investigated. A modulation of the MEL1 gene expression has been observed depending on growth condition, with a maximum of enzyme productivity during a transient physiological phase corresponding to the early stationary phase. Our experiments demonstrate that the highest α-galactosidase productivity observed in the early stationary phase of growth is not associated with a starvation condition, but it is coupled to a particular growth phase. The increase of specific activity of the enzyme occurs mainly for periplasmic and intracellular fractions while the amount of enzyme secreted in the medium remains fairly constant. © 1989.
PORRO, D., MARTEGANI, E., & RANZI, B. (1989). GROWTH-PHASE MODULATION OF THE PRODUCTIVITY OF ALPHA-GALACTOSIDASE IN BUDDING YEAST CULTURES. JOURNAL OF BIOTECHNOLOGY, 12(1), 71-78 [10.1016/0168-1656(89)90130-2].
Citazione: | PORRO, D., MARTEGANI, E., & RANZI, B. (1989). GROWTH-PHASE MODULATION OF THE PRODUCTIVITY OF ALPHA-GALACTOSIDASE IN BUDDING YEAST CULTURES. JOURNAL OF BIOTECHNOLOGY, 12(1), 71-78 [10.1016/0168-1656(89)90130-2]. | |
Tipo: | Articolo in rivista - Articolo scientifico | |
Carattere della pubblicazione: | Scientifica | |
Titolo: | GROWTH-PHASE MODULATION OF THE PRODUCTIVITY OF ALPHA-GALACTOSIDASE IN BUDDING YEAST CULTURES | |
Autori: | PORRO, D; MARTEGANI, E; RANZI, B | |
Autori: | ||
Data di pubblicazione: | 1989 | |
Lingua: | English | |
Rivista: | JOURNAL OF BIOTECHNOLOGY | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/0168-1656(89)90130-2 | |
Appare nelle tipologie: | 01 - Articolo su rivista |