The complete utilization of raffinose and melibiose by Saccharomyces cerevisiae requires the synthesis and secretion of an α-galactosidase encoded by a single MEL1 gene. The influence of culture conditions on the productivity of α-galactosidase in batch growth of yeast cells has been investigated. A modulation of the MEL1 gene expression has been observed depending on growth condition, with a maximum of enzyme productivity during a transient physiological phase corresponding to the early stationary phase. Our experiments demonstrate that the highest α-galactosidase productivity observed in the early stationary phase of growth is not associated with a starvation condition, but it is coupled to a particular growth phase. The increase of specific activity of the enzyme occurs mainly for periplasmic and intracellular fractions while the amount of enzyme secreted in the medium remains fairly constant. © 1989.

Porro, D., Martegani, E., Ranzi, B. (1989). GROWTH-PHASE MODULATION OF THE PRODUCTIVITY OF ALPHA-GALACTOSIDASE IN BUDDING YEAST CULTURES. JOURNAL OF BIOTECHNOLOGY, 12(1), 71-78 [10.1016/0168-1656(89)90130-2].

GROWTH-PHASE MODULATION OF THE PRODUCTIVITY OF ALPHA-GALACTOSIDASE IN BUDDING YEAST CULTURES

PORRO, DANILO;MARTEGANI, ENZO;
1989

Abstract

The complete utilization of raffinose and melibiose by Saccharomyces cerevisiae requires the synthesis and secretion of an α-galactosidase encoded by a single MEL1 gene. The influence of culture conditions on the productivity of α-galactosidase in batch growth of yeast cells has been investigated. A modulation of the MEL1 gene expression has been observed depending on growth condition, with a maximum of enzyme productivity during a transient physiological phase corresponding to the early stationary phase. Our experiments demonstrate that the highest α-galactosidase productivity observed in the early stationary phase of growth is not associated with a starvation condition, but it is coupled to a particular growth phase. The increase of specific activity of the enzyme occurs mainly for periplasmic and intracellular fractions while the amount of enzyme secreted in the medium remains fairly constant. © 1989.
Articolo in rivista - Articolo scientifico
ALPHA-GALACTOSIDASE, BUDDING YEAST
English
71
78
8
Porro, D., Martegani, E., Ranzi, B. (1989). GROWTH-PHASE MODULATION OF THE PRODUCTIVITY OF ALPHA-GALACTOSIDASE IN BUDDING YEAST CULTURES. JOURNAL OF BIOTECHNOLOGY, 12(1), 71-78 [10.1016/0168-1656(89)90130-2].
Porro, D; Martegani, E; Ranzi, B
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/33270
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