Saccharomyces cerevisiae cells transformed with plasmids bearing the lacZ gene from Escherichia coli, under the control of the inducible GAL1-10/CYC1 promoter, produce the highest amount of β-galactosidase during a transient physiological condition corresponding to the early stationary phase of growth. This enhanced enzyme expression is characteristic of active cycling cells down-modulated by the nutrients. By increasing the dosage of the GAL4 gene, after transformation of yeast cells with a multicopy plasmid bearing the GAL4 gene, a positive but limited enhancement of enzyme expression is induced

Lotti, M., Porro, D., Martegani, E., Alberghina, L. (1988). Physiological and genetic modulation of inducible expression of Escherichia coli β-galactosidase in Saccharomyces cerevisiae. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 28(2), 160-165 [10.1007/BF00694305].

Physiological and genetic modulation of inducible expression of Escherichia coli β-galactosidase in Saccharomyces cerevisiae

LOTTI, MARINA;PORRO, DANILO;MARTEGANI, ENZO;ALBERGHINA, LILIA
1988

Abstract

Saccharomyces cerevisiae cells transformed with plasmids bearing the lacZ gene from Escherichia coli, under the control of the inducible GAL1-10/CYC1 promoter, produce the highest amount of β-galactosidase during a transient physiological condition corresponding to the early stationary phase of growth. This enhanced enzyme expression is characteristic of active cycling cells down-modulated by the nutrients. By increasing the dosage of the GAL4 gene, after transformation of yeast cells with a multicopy plasmid bearing the GAL4 gene, a positive but limited enhancement of enzyme expression is induced
Articolo in rivista - Articolo scientifico
ESCHERICHIA-COLI, BETA-GALACTOSIDASE, SACCHAROMYCES-CEREVISIAE
English
160
165
6
Lotti, M., Porro, D., Martegani, E., Alberghina, L. (1988). Physiological and genetic modulation of inducible expression of Escherichia coli β-galactosidase in Saccharomyces cerevisiae. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 28(2), 160-165 [10.1007/BF00694305].
Lotti, M; Porro, D; Martegani, E; Alberghina, L
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/33265
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