Ecto-5'-nucleotidase (E5'N) is an extracellular enzyme forming anti-inflammatory and immunosuppressive adenosine. We evaluated whether confrontation of pig heart and endothelial cells with human blood changes the activity of E5'N. Pig hearts were perfused ex vivo with fresh human blood for 4 h. Pig aortic endothelial cells (PAEC) were incubated in vitro with human plasma for 3 h. Ex vivo perfusion of pig heart with fresh human blood resulted in a decrease in E5'N activity to 62% and 61% of initial in wild-type and transgenic pig hearts, respectively. PAEC activity of E5'N decreased to 71% and 50% of initial after 3 h exposure to heat-inactivated and active complement human plasma, respectively, while it remained constant in controls. Pig heart activity of E5'N decreased following exposure to human blood, which may affect adenosine production and exacerbate hyperacute and vascular rejection.

Khalpey, Z., Kalsi, K., Yuen, A., Karbowska, J., Kochan, Z., Slominska, E., et al. (2005). Exposure to human blood inactivates swine endothelial ecto-5 '-nucleotidase. NUCLEOSIDES, NUCLEOTIDES & NUCLEIC ACIDS, 24(4), 271-274 [10.1081/NCN-59707].

Exposure to human blood inactivates swine endothelial ecto-5 '-nucleotidase

LAVITRANO, MARIALUISA;
2005

Abstract

Ecto-5'-nucleotidase (E5'N) is an extracellular enzyme forming anti-inflammatory and immunosuppressive adenosine. We evaluated whether confrontation of pig heart and endothelial cells with human blood changes the activity of E5'N. Pig hearts were perfused ex vivo with fresh human blood for 4 h. Pig aortic endothelial cells (PAEC) were incubated in vitro with human plasma for 3 h. Ex vivo perfusion of pig heart with fresh human blood resulted in a decrease in E5'N activity to 62% and 61% of initial in wild-type and transgenic pig hearts, respectively. PAEC activity of E5'N decreased to 71% and 50% of initial after 3 h exposure to heat-inactivated and active complement human plasma, respectively, while it remained constant in controls. Pig heart activity of E5'N decreased following exposure to human blood, which may affect adenosine production and exacerbate hyperacute and vascular rejection.
Articolo in rivista - Articolo scientifico
ecto-5 '-nucleotidease; xenotransplantation; nucleotides; endothelial cells; complement
English
2005
24
4
271
274
none
Khalpey, Z., Kalsi, K., Yuen, A., Karbowska, J., Kochan, Z., Slominska, E., et al. (2005). Exposure to human blood inactivates swine endothelial ecto-5 '-nucleotidase. NUCLEOSIDES, NUCLEOTIDES & NUCLEIC ACIDS, 24(4), 271-274 [10.1081/NCN-59707].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/3070
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