BACKGROUND: Lower risk of digestive tract cancer development has been associated with polyphenol intake. Bactris guineensis is an edible endemic palm that grows in Central and South America. OBJECTIVE: This study performs a phenolic characterization of Bactris guineensis and evaluates the bioactivity of this fruit. METHODS: The phenolic compounds of B. guineensis were characterized by HPLC-UV-HRMS analyses and the antioxidant activity was measured by chemical and cellular methods. Additionally, cytotoxicity of B. guineensis polyphenols was performed on 4 cancer cell lines and the pro-apoptotic effect was evaluated by flow cytometry using annexin staining. RESULTS: The major phenolic compounds of B. guineensis were proanthocyanidins. The extract IC50 for DPPH was 3.3±0.2 μg/mL and for induced intracellular ROS was 153±13 μg/mL. MTT cytotoxic assays demonstrate IC50 values between 16.6 and 24.9 μg/mL for the colon and hepatic adenocarcinomas, with high selectivity effects towards cancer cells compared to non-tumor cells. A 20 to 50% early apoptotic effect was observed in cancer cells lines by Annexin/PI staining. CONCLUSIONS: B. guineensis evidenced an important radical scavenging activity and a strong cytotoxic activity against hepatic and colorectal carcinoma cells, showing better values than procyanidin extracts from other fruits previously described.
Quesada, M., Azofeifa, G., Campone, L., Pagano, I., Perez, A., Cortes, C., et al. (2020). Bactris guineensis (Arecaceae) extract: Polyphenol characterization, antioxidant capacity and cytotoxicity against cancer cell lines. JOURNAL OF BERRY RESEARCH, 10(3), 329-344 [10.3233/JBR-190449].
Bactris guineensis (Arecaceae) extract: Polyphenol characterization, antioxidant capacity and cytotoxicity against cancer cell lines
Campone L.;
2020
Abstract
BACKGROUND: Lower risk of digestive tract cancer development has been associated with polyphenol intake. Bactris guineensis is an edible endemic palm that grows in Central and South America. OBJECTIVE: This study performs a phenolic characterization of Bactris guineensis and evaluates the bioactivity of this fruit. METHODS: The phenolic compounds of B. guineensis were characterized by HPLC-UV-HRMS analyses and the antioxidant activity was measured by chemical and cellular methods. Additionally, cytotoxicity of B. guineensis polyphenols was performed on 4 cancer cell lines and the pro-apoptotic effect was evaluated by flow cytometry using annexin staining. RESULTS: The major phenolic compounds of B. guineensis were proanthocyanidins. The extract IC50 for DPPH was 3.3±0.2 μg/mL and for induced intracellular ROS was 153±13 μg/mL. MTT cytotoxic assays demonstrate IC50 values between 16.6 and 24.9 μg/mL for the colon and hepatic adenocarcinomas, with high selectivity effects towards cancer cells compared to non-tumor cells. A 20 to 50% early apoptotic effect was observed in cancer cells lines by Annexin/PI staining. CONCLUSIONS: B. guineensis evidenced an important radical scavenging activity and a strong cytotoxic activity against hepatic and colorectal carcinoma cells, showing better values than procyanidin extracts from other fruits previously described.
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