The ability to introduce controlled modifications of the genome of animals represents an important tool for biomedical and veterinary research. Among transgenic techniques, we describe here the sperm-mediated gene transfer method that is based on the spontaneous ability of sperm cells to bind and internalize exogenous DNA and to carry it to the oocyte during fertilization, producing genetically modified animals.
Lavitrano, M., Farina, L., Cerrito, M., Giovannoni, R. (2020). Sperm-Mediated Genetic Modifications. In M. Larson (a cura di), Transgenic Mouse : Methods and Protocols (pp. 125-132). Humana Press Inc. [10.1007/978-1-4939-9837-1_11].
Sperm-Mediated Genetic Modifications
Lavitrano, M
;Farina, L;Cerrito, MGMembro del Collaboration Group
;Giovannoni, RMembro del Collaboration Group
2020
Abstract
The ability to introduce controlled modifications of the genome of animals represents an important tool for biomedical and veterinary research. Among transgenic techniques, we describe here the sperm-mediated gene transfer method that is based on the spontaneous ability of sperm cells to bind and internalize exogenous DNA and to carry it to the oocyte during fertilization, producing genetically modified animals.
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