Generation of 3' single-stranded DNA (ssDNA) at the ends of a double-strand break (DSB) is essential to initiate repair by homology-directed mechanisms. Here we describe a Southern blot-based method to visualize the generation of ssDNA at the ends of site-specific DSBs generated in the Saccharomyces cerevisiae genome.
Casari, E., Gobbini, E., Clerici, M., Longhese, M. (2021). Resection of a DNA Double-Strand Break by Alkaline Gel Electrophoresis and Southern Blotting. In Homologous Recombination (pp. 33-45). 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA : Humana Press Inc. [10.1007/978-1-0716-0644-5_3].
Resection of a DNA Double-Strand Break by Alkaline Gel Electrophoresis and Southern Blotting
Casari, E;Gobbini, E;Clerici, M;Longhese, MP
2021
Abstract
Generation of 3' single-stranded DNA (ssDNA) at the ends of a double-strand break (DSB) is essential to initiate repair by homology-directed mechanisms. Here we describe a Southern blot-based method to visualize the generation of ssDNA at the ends of site-specific DSBs generated in the Saccharomyces cerevisiae genome.
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