Microorganisms employed in industrial fermentation processes are often subjected to a variety of stresses that negatively affect growth, production and productivity. Therefore, stress robustness is an important property for their application. Reactive Oxygen Species (ROS) accumulation is a common denominator to a lot of these stress factors. Ascorbic acid (L-AA) acts as ROS scavenger, thus potentially protecting cells from harmful oxidative products. We have previously reported the development of Saccharomyces cerevisiae strains able to produce L-AA. This was obtained by expressing the known plant pathway genes and by complementing the missing step with an animal activity. Here, we show that L-AA accumulation inside yeast cells can be improved by expressing the complete biosynthetic plant pathway and, even further, by recycling its oxidation products. These new strains can be seen in a double perspective of exploitation: as novel organisms for vitamin C production and as novel cell factories for industrial processes

Fossati, T., Solinas, N., Porro, D., Branduardi, P. (2011). L-ascorbic acid producing yeasts learn from plants how to recycle it. METABOLIC ENGINEERING, 13(2), 177-185 [10.1016/j.ymben.2010.12.007].

L-ascorbic acid producing yeasts learn from plants how to recycle it

FOSSATI, TIZIANA;PORRO, DANILO;BRANDUARDI, PAOLA
2011

Abstract

Microorganisms employed in industrial fermentation processes are often subjected to a variety of stresses that negatively affect growth, production and productivity. Therefore, stress robustness is an important property for their application. Reactive Oxygen Species (ROS) accumulation is a common denominator to a lot of these stress factors. Ascorbic acid (L-AA) acts as ROS scavenger, thus potentially protecting cells from harmful oxidative products. We have previously reported the development of Saccharomyces cerevisiae strains able to produce L-AA. This was obtained by expressing the known plant pathway genes and by complementing the missing step with an animal activity. Here, we show that L-AA accumulation inside yeast cells can be improved by expressing the complete biosynthetic plant pathway and, even further, by recycling its oxidation products. These new strains can be seen in a double perspective of exploitation: as novel organisms for vitamin C production and as novel cell factories for industrial processes
Articolo in rivista - Articolo scientifico
L-ascorbic acid; robustness; plant; DHAR
English
2011
13
2
177
185
none
Fossati, T., Solinas, N., Porro, D., Branduardi, P. (2011). L-ascorbic acid producing yeasts learn from plants how to recycle it. METABOLIC ENGINEERING, 13(2), 177-185 [10.1016/j.ymben.2010.12.007].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/27992
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