Matrix-Assisted Laser Desorption/Ionization (MALDI)-Mass Spectrometry imaging (MSI) has been applied in various diseases aimed to biomarkers discovery. In this study diagnosis and prognosis of Hashimoto Thyroiditis (HT) in cytopathology by MALDI-MSI has been investigated. Specimens from a routine series of subjects who underwent UltraSound-guided thyroid Fine Needle Aspirations (FNAs) were used. The molecular classifier trained in a previous study was modified to include HT as a separate entity in the group of benign lesions, in the diagnostic proteomic triage of thyroid nodules. The statistical analysis confirmed the existence of signals that HT shares with hyperplastic lesions and others that are specific and characterize this subgroup. Statistically relevant HT-related peaks were included in the model. Then, the discriminatory capability of the classifier was tested in a second validation phase, showing a good agreement with cytological diagnoses. The possibility to overlap the molecular signatures of both the lymphocytes and epithelial cells components (ROIs or pixel-by-pixel analysis) confirmed the composite proteomic background of HT. These results open the way to their possible translation as alternative serum biomarkers of this autoimmune condition.
Capitoli, G., Piga, I., Clerici, F., Brambilla, V., Mahajneh, A., Leni, D., et al. (2020). Analysis of Hashimoto's thyroiditis on fine needle aspiration samples by MALDI-Imaging. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1868(11).
|Citazione:||Capitoli, G., Piga, I., Clerici, F., Brambilla, V., Mahajneh, A., Leni, D., et al. (2020). Analysis of Hashimoto's thyroiditis on fine needle aspiration samples by MALDI-Imaging. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1868(11).|
|Tipo:||Articolo in rivista - Articolo scientifico|
|Carattere della pubblicazione:||Scientifica|
|Presenza di un coautore afferente ad Istituzioni straniere:||No|
|Titolo:||Analysis of Hashimoto's thyroiditis on fine needle aspiration samples by MALDI-Imaging|
|Autori:||Capitoli, G; Piga, I; Clerici, F; Brambilla, V; Mahajneh, A; Leni, D; Garancini, M; Pincelli, A; L'Imperio, V; Galimberti, S; Magni, F; Pagni, F|
MAGNI, FULVIO (Corresponding)
PAGNI, FABIO (Corresponding)
|Data di pubblicazione:||2020|
|Rivista:||BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.bbapap.2020.140481|
|Appare nelle tipologie:||01 - Articolo su rivista|