Synthetic 3D extracellular matrices (ECMs) find application in cell studies, regenerative medicine, and drug discovery. While cells cultured in a monolayer may exhibit unnatural behavior and develop very different phenotypes and genotypes than in vivo, great efforts in materials chemistry have been devoted to reproducing in vitro behavior in in vivo cell microenvironments. This requires fine-tuning the biochemical and structural actors in synthetic ECMs. This review will present the fundamentals of the ECM, cover the chemical and structural features of the scaffolds used to generate ECM mimics, discuss the nature of the signaling biomolecules required and exploited to generate bioresponsive cell microenvironments able to induce a specific cell fate, and highlight the synthetic strategies involved in creating functional 3D ECM mimics.

Nicolas, J., Magli, S., Rabbachin, L., Sampaolesi, S., Nicotra, F., Russo, L. (2020). 3D Extracellular Matrix Mimics: Fundamental Concepts and Role of Materials Chemistry to Influence Stem Cell Fate. BIOMACROMOLECULES, 21(6), 1968-1994 [10.1021/acs.biomac.0c00045].

3D Extracellular Matrix Mimics: Fundamental Concepts and Role of Materials Chemistry to Influence Stem Cell Fate

Sofia Magli;Susanna Sampaolesi;Francesco Nicotra;Laura Russo
2020

Abstract

Synthetic 3D extracellular matrices (ECMs) find application in cell studies, regenerative medicine, and drug discovery. While cells cultured in a monolayer may exhibit unnatural behavior and develop very different phenotypes and genotypes than in vivo, great efforts in materials chemistry have been devoted to reproducing in vitro behavior in in vivo cell microenvironments. This requires fine-tuning the biochemical and structural actors in synthetic ECMs. This review will present the fundamentals of the ECM, cover the chemical and structural features of the scaffolds used to generate ECM mimics, discuss the nature of the signaling biomolecules required and exploited to generate bioresponsive cell microenvironments able to induce a specific cell fate, and highlight the synthetic strategies involved in creating functional 3D ECM mimics.
Articolo in rivista - Articolo scientifico
Cells; Peptides and proteins; Extracellular matrix; Cell physiology; Receptors;
English
31-mar-2020
2020
21
6
1968
1994
none
Nicolas, J., Magli, S., Rabbachin, L., Sampaolesi, S., Nicotra, F., Russo, L. (2020). 3D Extracellular Matrix Mimics: Fundamental Concepts and Role of Materials Chemistry to Influence Stem Cell Fate. BIOMACROMOLECULES, 21(6), 1968-1994 [10.1021/acs.biomac.0c00045].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/277617
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