Three propolis samples were collected from different regions of Ecuador (Quito, Guayaquil and Cotacachi) and their methanolic extracts were prepared. Preliminary information supplied by TLC and NMR data, allowed us to define two main types of propolis: Cotacachi propoli sample (CPS), rich in flavonoids and Quito and Guayaquil samples (QPS and GPS) containing triterpenic alcohols and acetyl triterpenes as the main constituents. Two different approaches based on RP-HPLC preparative procedure and NMR structural determination (CPS) and GC–MS analysis (QPS and GPS) were successfully used for the chemical characterization of their major compounds. All three propolis extracts were able to inhibit Leishmania amazonensis growth but propolis sample rich in flavonoids was the most active (IC50 = 17.1 ± 1.7 μg/mL). In the literature this is the first study on propolis from Ecuador.

Cuesta-Rubio, O., Campo Fernandez, M., Marquez Hernandez, I., Jaramillo, C., Gonzalez, V., Montes De Oca Porto, R., et al. (2017). Chemical profile and anti-leishmanial activity of three Ecuadorian propolis samples from Quito, Guayaquil and Cotacachi regions. FITOTERAPIA, 120, 177-183 [10.1016/j.fitote.2017.06.016].

Chemical profile and anti-leishmanial activity of three Ecuadorian propolis samples from Quito, Guayaquil and Cotacachi regions

Campone L.;
2017

Abstract

Three propolis samples were collected from different regions of Ecuador (Quito, Guayaquil and Cotacachi) and their methanolic extracts were prepared. Preliminary information supplied by TLC and NMR data, allowed us to define two main types of propolis: Cotacachi propoli sample (CPS), rich in flavonoids and Quito and Guayaquil samples (QPS and GPS) containing triterpenic alcohols and acetyl triterpenes as the main constituents. Two different approaches based on RP-HPLC preparative procedure and NMR structural determination (CPS) and GC–MS analysis (QPS and GPS) were successfully used for the chemical characterization of their major compounds. All three propolis extracts were able to inhibit Leishmania amazonensis growth but propolis sample rich in flavonoids was the most active (IC50 = 17.1 ± 1.7 μg/mL). In the literature this is the first study on propolis from Ecuador.
Articolo in rivista - Articolo scientifico
Antileishmanial activity; Ecuadorian propolis; Flavonoids; GC–MS; NMR; Triterpenoid;
Antileishmanial activity; Ecuadorian propolis; Flavonoids; GC–MS; NMR; Triterpenoid
English
2017
120
177
183
none
Cuesta-Rubio, O., Campo Fernandez, M., Marquez Hernandez, I., Jaramillo, C., Gonzalez, V., Montes De Oca Porto, R., et al. (2017). Chemical profile and anti-leishmanial activity of three Ecuadorian propolis samples from Quito, Guayaquil and Cotacachi regions. FITOTERAPIA, 120, 177-183 [10.1016/j.fitote.2017.06.016].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/263759
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