1. The delta-endotoxin of Bacillus thuringiensis subsp. aizawai inhibits in a dose-dependent manner the K+-driven accumulation of histidine and lysine as well as their equilibrative uptake into brush border membrane vesicles from the midgut of Bombyx mori larvae. 2. The decrease of uptake in the absence of a K+-gradient is neither due to a leakage of the labelled substrate from the vesicles nor to a reduction of the osmotically active space available, as a result of a detergent-like effect of the toxin. 3. The toxin acts as a non competitive inhibitor of the K+/amino acid cotransporters of the larval midgut of Lepidoptera, with no preference for a specific transport system
Giordana, B., Tasca, M., Villa, M., Chiantore, C., Hanozet, G., Parenti, P. (1993). Bacillus-thuringiensis subsp aizawai delta-endotoxin inhibits the k+/amino acid cotransporters of lepidopteran larval midgut. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. C, COMPARATIVE PHARMACOLOGY AND TOXICOLOGY, 106(2), 403-407 [10.1016/0742-8413(93)90153-C].
Bacillus-thuringiensis subsp aizawai delta-endotoxin inhibits the k+/amino acid cotransporters of lepidopteran larval midgut
PARENTI, PAOLO
1993
Abstract
1. The delta-endotoxin of Bacillus thuringiensis subsp. aizawai inhibits in a dose-dependent manner the K+-driven accumulation of histidine and lysine as well as their equilibrative uptake into brush border membrane vesicles from the midgut of Bombyx mori larvae. 2. The decrease of uptake in the absence of a K+-gradient is neither due to a leakage of the labelled substrate from the vesicles nor to a reduction of the osmotically active space available, as a result of a detergent-like effect of the toxin. 3. The toxin acts as a non competitive inhibitor of the K+/amino acid cotransporters of the larval midgut of Lepidoptera, with no preference for a specific transport system
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