We conducted a proteomics study in order to detect the proteomic method which provides the most complete characterization of the proteins of rice milk. In particular, we compared the results obtained from LC-MS/MS after protein precipitation with acetone or TCA, as well as the results obtained from LC-MS/MS after protein prefractionation based on SDS-PAGE (GeLC-MS/MS) or ProteoMiner™ technology (ProteoMiner-LC-MS/MS), and after peptide prefractionation based on IEF (pIEF-LC-MS/MS). A total of 158 protein species have been detect in rice milk. The physical-chemical analysis and classification of the identified proteins were also reported. In particular, we showed that pIEF-LC-MS/MS method led to a significant increase in the proteome coverage, allowing the identification of a total of 96 proteins of milk rice. This study demonstrates the utility of a prefractionation step based on pIEF before the shotgun proteomic analysis and offers an in-depth insight into the rice milk proteome.

Manfredi, M., Brandi, J., Conte, E., Pidutti, P., Gosetti, F., Robotti, E., et al. (2017). IEF peptide fractionation method combined to shotgun proteomics enhances the exploration of rice milk proteome. ANALYTICAL BIOCHEMISTRY, 537, 72-77 [10.1016/j.ab.2017.08.021].

IEF peptide fractionation method combined to shotgun proteomics enhances the exploration of rice milk proteome

Gosetti, F;
2017

Abstract

We conducted a proteomics study in order to detect the proteomic method which provides the most complete characterization of the proteins of rice milk. In particular, we compared the results obtained from LC-MS/MS after protein precipitation with acetone or TCA, as well as the results obtained from LC-MS/MS after protein prefractionation based on SDS-PAGE (GeLC-MS/MS) or ProteoMiner™ technology (ProteoMiner-LC-MS/MS), and after peptide prefractionation based on IEF (pIEF-LC-MS/MS). A total of 158 protein species have been detect in rice milk. The physical-chemical analysis and classification of the identified proteins were also reported. In particular, we showed that pIEF-LC-MS/MS method led to a significant increase in the proteome coverage, allowing the identification of a total of 96 proteins of milk rice. This study demonstrates the utility of a prefractionation step based on pIEF before the shotgun proteomic analysis and offers an in-depth insight into the rice milk proteome.
Articolo in rivista - Articolo scientifico
Isoelectrofocusing; Milk; Proteome; Shotgun proteomics;
Isoelectrofocusing; Milk; Proteome; Shotgun proteomics
English
2017
537
72
77
reserved
Manfredi, M., Brandi, J., Conte, E., Pidutti, P., Gosetti, F., Robotti, E., et al. (2017). IEF peptide fractionation method combined to shotgun proteomics enhances the exploration of rice milk proteome. ANALYTICAL BIOCHEMISTRY, 537, 72-77 [10.1016/j.ab.2017.08.021].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/253188
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