Background: Bruton’s tyrosine kinase (BTK) is involved in the immune response and its deficiency impairs B cell maturation. We evaluated the expression of a novel BTK isoform, p65BTK, in colorectal cancer (CRC), to identify its impact on survival. Materials and Methods: This retrospective study evaluated 87 consecutive stage III CRC patients treated at the National Cancer Institute of Aviano (1999-2017). Multiple specimens were collected and analyzed for staining intensity and percentage of tumor cells positive for p65BTK. Prognostic impact was tested by univariate Cox regression analysis. Results: After a median follow-up of 82.59 months, median disease-free survival (DFS) and overall survival (OS) were 11.67 months and 31.33 months, respectively. Interestingly, 10% of patients did not express p65BTK. For the immunohistochemistry IHC intensity 1, the best cutoff point was 1% of p65BTK positivity; for IHC intensity 2, it was 50%; and for IHC intensity 3, it was 80%. Through univariate analysis, patients with highly expressed p65BTK (IHC intensity 3 and ≥80%) were shown to have the worst prognosis in terms of DFS (HR: 6.23; p = 0.005; 95% C.I. 1.75-22.79) and OS (HR: 2.54; p = 0.025; 95% C.I. 1.12-5.76). Conclusions: p65BTK is frequently expressed in CRC and, if highly expressed, is an unfavourable prognostic factor. However, further confirmation is needed and its potential targeting needs to be studied

Basile, D., Gerratana, L., Buonadonna, A., Garattini, S., Perin, T., Grassilli, E., et al. (2019). Role of Bruton's Tyrosine Kinase in Stage III Colorectal Cancer. CANCERS, 11(6) [10.3390/cancers11060880].

Role of Bruton's Tyrosine Kinase in Stage III Colorectal Cancer

Grassilli, Emanuela;Cerrito, Maria Grazia;Lavitrano, Marialuisa;
2019

Abstract

Background: Bruton’s tyrosine kinase (BTK) is involved in the immune response and its deficiency impairs B cell maturation. We evaluated the expression of a novel BTK isoform, p65BTK, in colorectal cancer (CRC), to identify its impact on survival. Materials and Methods: This retrospective study evaluated 87 consecutive stage III CRC patients treated at the National Cancer Institute of Aviano (1999-2017). Multiple specimens were collected and analyzed for staining intensity and percentage of tumor cells positive for p65BTK. Prognostic impact was tested by univariate Cox regression analysis. Results: After a median follow-up of 82.59 months, median disease-free survival (DFS) and overall survival (OS) were 11.67 months and 31.33 months, respectively. Interestingly, 10% of patients did not express p65BTK. For the immunohistochemistry IHC intensity 1, the best cutoff point was 1% of p65BTK positivity; for IHC intensity 2, it was 50%; and for IHC intensity 3, it was 80%. Through univariate analysis, patients with highly expressed p65BTK (IHC intensity 3 and ≥80%) were shown to have the worst prognosis in terms of DFS (HR: 6.23; p = 0.005; 95% C.I. 1.75-22.79) and OS (HR: 2.54; p = 0.025; 95% C.I. 1.12-5.76). Conclusions: p65BTK is frequently expressed in CRC and, if highly expressed, is an unfavourable prognostic factor. However, further confirmation is needed and its potential targeting needs to be studied
Articolo in rivista - Articolo scientifico
BTK; Bruton’s tyrosine kinase; colon cancer
English
Basile, D., Gerratana, L., Buonadonna, A., Garattini, S., Perin, T., Grassilli, E., et al. (2019). Role of Bruton's Tyrosine Kinase in Stage III Colorectal Cancer. CANCERS, 11(6) [10.3390/cancers11060880].
Basile, D; Gerratana, L; Buonadonna, A; Garattini, S; Perin, T; Grassilli, E; Miolo, G; Cerrito, M; Belluco, C; Bertola, G; De Paoli, A; Cannizzaro, R; Lavitrano, M; Puglisi, F; Canzonieri, V
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/241829
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