The biological effects induced in human pulmonary cells by atmospheric particles collected in Milan have been analysed according to their dimension and season of sampling. Summer and winter PM1, PM2.5 and PM10 were chemically characterized and used for exposure of pulmonary cultured cell lines. Cell viability, proinflammatory cytokine expression, cell cycle modifications and cytochrome P450 CYP1B1 expression were analysed. Chemical characterization showed that summer fractions had a higher endotoxin content and were enriched in metals while winter PMs in PAHs. In line with this aspect, summer PM10 induced stronger cytotoxic and inflammatory effects in comparison with the other fractions. Winter fine PMs provoked a cell cycle arrest in G2/M phases and remarkable cytochrome P450 CYP1B1 activation, suggesting a potential genotoxic effect.
Longhin, E., Pezzolato, E., Mantecca, P., Gualtieri, M., Bolzacchini, E., Camatini, M. (2010). Biological effects of Milan PM: the role of particles dimension and season of sampling. In AAAS10: ADVANCED ATMOSPHERIC AEROSOL SYMPOSIUM (pp.23-28) [10.3303/CET1022003].
Biological effects of Milan PM: the role of particles dimension and season of sampling
LONGHIN, ELEONORA MARTA;MANTECCA, PARIDE;GUALTIERI, MAURIZIO;BOLZACCHINI, EZIO;CAMATINI, MARINA CARLA
2010
Abstract
The biological effects induced in human pulmonary cells by atmospheric particles collected in Milan have been analysed according to their dimension and season of sampling. Summer and winter PM1, PM2.5 and PM10 were chemically characterized and used for exposure of pulmonary cultured cell lines. Cell viability, proinflammatory cytokine expression, cell cycle modifications and cytochrome P450 CYP1B1 expression were analysed. Chemical characterization showed that summer fractions had a higher endotoxin content and were enriched in metals while winter PMs in PAHs. In line with this aspect, summer PM10 induced stronger cytotoxic and inflammatory effects in comparison with the other fractions. Winter fine PMs provoked a cell cycle arrest in G2/M phases and remarkable cytochrome P450 CYP1B1 activation, suggesting a potential genotoxic effect.
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