Introduction: The high concentration of particulate matter (PM10, PM2.5 and PM1) in Milan is a cause of health concern. This study investigates the biological effects of PM10, PM2.5 and PM1 sampled in Milan during summer and winter seasons for the evidence of a seasonal difference in PMs chemical and microbiological composition. Results: The winter PMs presented higher levels of PAHs in comparison to the summer ones, which contained a greater amount of mineral dust elements and biological components such as gram-negative bacteria (LAL test). PM biological effects were analysed on the human bronchial epithelial cell line BEAS-2B and the monocytes cell line THP-1. Winter PMs were toxic in BEAS-2B and THP-1 as assessed by PI/Hoechst staining, while summer PMs induced a higher pro-inflammatory response. Summer PM10 induced IL-8 release in both the cell lines. Interestingly summer PM10 induced in THP-1 cells an increase in IL-1b expression. Moreover winter PMs were able to induce a G2/M arrest in BEAS-2B and THP-1 cell cycle. Conclusions: These results evidence a correlation between the PM chemical/biogenic composition and the biological effects. The higher content of LPS deriving from the gram-negative bacteria correlates with the higher pro-inflammatory potency of the summer PMs, while the higher content of PAHs in winter might be responsible for the increased cytotoxicity and cell cycle arrest
Gualtieri, M., Longhin, E., Pezzolato, E., Mantecca, P., Molteni, E., Holme, J., et al. (2010). In vitro effects of summer and winter Milan particulate matter. In Abstracts of the XII International Congress of Toxicology (pp.65-65). Elsevier [10.1016/j.toxlet.2010.03.249].
In vitro effects of summer and winter Milan particulate matter
GUALTIERI, MAURIZIO;LONGHIN, ELEONORA MARTA;MANTECCA, PARIDE;CAMATINI, MARINA CARLA
2010
Abstract
Introduction: The high concentration of particulate matter (PM10, PM2.5 and PM1) in Milan is a cause of health concern. This study investigates the biological effects of PM10, PM2.5 and PM1 sampled in Milan during summer and winter seasons for the evidence of a seasonal difference in PMs chemical and microbiological composition. Results: The winter PMs presented higher levels of PAHs in comparison to the summer ones, which contained a greater amount of mineral dust elements and biological components such as gram-negative bacteria (LAL test). PM biological effects were analysed on the human bronchial epithelial cell line BEAS-2B and the monocytes cell line THP-1. Winter PMs were toxic in BEAS-2B and THP-1 as assessed by PI/Hoechst staining, while summer PMs induced a higher pro-inflammatory response. Summer PM10 induced IL-8 release in both the cell lines. Interestingly summer PM10 induced in THP-1 cells an increase in IL-1b expression. Moreover winter PMs were able to induce a G2/M arrest in BEAS-2B and THP-1 cell cycle. Conclusions: These results evidence a correlation between the PM chemical/biogenic composition and the biological effects. The higher content of LPS deriving from the gram-negative bacteria correlates with the higher pro-inflammatory potency of the summer PMs, while the higher content of PAHs in winter might be responsible for the increased cytotoxicity and cell cycle arrest
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
