Diesel exhaust particles (DEP) are responsible for both respiratory and cardiovascular effects. However many questions are still unravelled and the mechanisms behind the health effects induced by the exposure to ultrafine particles (UFP) need further investigations. Furthermore, different emission sources can lead to diverse biological responses. In this perspective, here we have compared the effects of three DEPs, two standard reference materials (SRM 1650b and 2975) and one DEP directly sampled from a EuroIV vehicle without Diesel Particulate Filter (DPF). For the biological investigations, different in vitro lung models involving both epithelial and vascular endothelial cells, were used. Cell viability, oxidative stress, inflammation, DNA damage and endothelial activation markers were investigated at sub-cytotoxic DEP doses. The data obtained have shown that only DEP EuroIV, which had the major content of polycyclic aromatic hydrocarbons (PAHs) and metals, was able to induce oxidative stress, inflammation and consequent endothelial activation, as demonstrated by the expression of adhesion molecules (ICAM-1 and VCAM-1) and the release of inflammatory markers (IL-8) from endothelial cells. Standard reference materials were not effective under our experimental conditions. These data suggest that oxidative stress, endothelial activation and systemic inflammatory cytokines release are crucial events after DEP exposure and that the source of DEP emission, responsible of the particle chemical fingerprint, may have a key role in the resulting adverse biological outcomes.

Bengalli, R., Zerboni, A., Marchetti, S., Longhin, E., Priola, M., Camatini, M., et al. (2019). In vitro pulmonary and vascular effects induced by different diesel exhaust particles. TOXICOLOGY LETTERS, 306, 13-24 [10.1016/j.toxlet.2019.01.017].

In vitro pulmonary and vascular effects induced by different diesel exhaust particles

Bengalli R.
Co-primo
;
Zerboni A.
Co-primo
;
Marchetti S.
Secondo
;
Longhin E.;Camatini M.;Mantecca P.
Ultimo
2019

Abstract

Diesel exhaust particles (DEP) are responsible for both respiratory and cardiovascular effects. However many questions are still unravelled and the mechanisms behind the health effects induced by the exposure to ultrafine particles (UFP) need further investigations. Furthermore, different emission sources can lead to diverse biological responses. In this perspective, here we have compared the effects of three DEPs, two standard reference materials (SRM 1650b and 2975) and one DEP directly sampled from a EuroIV vehicle without Diesel Particulate Filter (DPF). For the biological investigations, different in vitro lung models involving both epithelial and vascular endothelial cells, were used. Cell viability, oxidative stress, inflammation, DNA damage and endothelial activation markers were investigated at sub-cytotoxic DEP doses. The data obtained have shown that only DEP EuroIV, which had the major content of polycyclic aromatic hydrocarbons (PAHs) and metals, was able to induce oxidative stress, inflammation and consequent endothelial activation, as demonstrated by the expression of adhesion molecules (ICAM-1 and VCAM-1) and the release of inflammatory markers (IL-8) from endothelial cells. Standard reference materials were not effective under our experimental conditions. These data suggest that oxidative stress, endothelial activation and systemic inflammatory cytokines release are crucial events after DEP exposure and that the source of DEP emission, responsible of the particle chemical fingerprint, may have a key role in the resulting adverse biological outcomes.
Articolo in rivista - Articolo scientifico
Diesel exhaust particles; Endothelial activation; In vitro toxicity; Inflammatory mediators; Air Pollutants; Blood Vessels; Cell Survival; DNA Damage; Endothelial Cells; Humans; Intercellular Adhesion Molecule-1; Lung; Metals; Oxidative Stress; Particle Size; Particulate Matter; Polycyclic Aromatic Hydrocarbons; Vascular Cell Adhesion Molecule-1; Vehicle Emissions
English
13
24
12
Bengalli, R., Zerboni, A., Marchetti, S., Longhin, E., Priola, M., Camatini, M., et al. (2019). In vitro pulmonary and vascular effects induced by different diesel exhaust particles. TOXICOLOGY LETTERS, 306, 13-24 [10.1016/j.toxlet.2019.01.017].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/240308
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