Binding of ligands to DNA gives rise to several relevant biological and biomedical effects. Here, through the use of atomic force microscopy (AFM), we studied the consequences of drug binding on the morphology of single DNA molecules. In particular, we quantitatively analyzed the effects of three different DNA-binding molecules (doxorubicin, ethidium bromide, and netropsin) that exert various pharmacologic and therapeutic effects. The results of this study show the consequences of intercalation and groove molecular binding on DNA conformation. These single-molecule measurements demonstrate morphological features that reflect the specific modes of drug-DNA interaction. This experimental approach may have implications in the design of therapeutically effective agents.

Cassina, V., Seruggia, D., Beretta, G., Salerno, D., Brogioli, D., Manzini, S., et al. (2011). Atomic force microscopy study of DNA conformation in the presence of drugs. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 40(1), 59-68 [10.1007/s00249-010-0627-6].

Atomic force microscopy study of DNA conformation in the presence of drugs

CASSINA, VALERIA
;
SALERNO, DOMENICO;BROGIOLI, DORIANO COSTANTINO;MANZINI, STEFANO;MANTEGAZZA, FRANCESCO
2011

Abstract

Binding of ligands to DNA gives rise to several relevant biological and biomedical effects. Here, through the use of atomic force microscopy (AFM), we studied the consequences of drug binding on the morphology of single DNA molecules. In particular, we quantitatively analyzed the effects of three different DNA-binding molecules (doxorubicin, ethidium bromide, and netropsin) that exert various pharmacologic and therapeutic effects. The results of this study show the consequences of intercalation and groove molecular binding on DNA conformation. These single-molecule measurements demonstrate morphological features that reflect the specific modes of drug-DNA interaction. This experimental approach may have implications in the design of therapeutically effective agents.
Articolo in rivista - Articolo scientifico
AFM, ligands
English
59
68
10
Cassina, V., Seruggia, D., Beretta, G., Salerno, D., Brogioli, D., Manzini, S., et al. (2011). Atomic force microscopy study of DNA conformation in the presence of drugs. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 40(1), 59-68 [10.1007/s00249-010-0627-6].
Cassina, V; Seruggia, D; Beretta, G; Salerno, D; Brogioli, D; Manzini, S; Zunino, F; Mantegazza, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/18357
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