Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is a unique analytical technology that explores the spatial distribution of biomolecules (proteins, lipids, and metabolites) directly in situ, thus integrating molecular and morphological information. In particular, MALDI-MSI of tissue represents a new stimulating perspective in the field of proteomics and facilitates the detection of specific proteomic indicators that are directly correlated with disease alterations. Here we describe the key experimental steps for the MALDI-MSI analysis of both fresh-frozen and formalin-fixed paraffin-embedded tissue, in particular for clinically orientated studies. Furthermore, we highlight how this approach has been employed in the study of renal diseases and can provide proteomic signatures of the most common glomerular diseases.
Smith, A., Mosele, N., L'Imperio, V., Pagni, F., Magni, F. (2018). Tissue MALDI Imaging. In A. Vlahou, H. Mischak, J. Zoidakis, F. Magni (a cura di), Integration of Omics Approaches and Systems Biology for Clinical Applications (pp. 156-172). John Wiley & Sons, Inc. [10.1002/9781119183952.ch9].
Tissue MALDI Imaging
Smith A.;L'Imperio V.;Pagni F.;Magni F.
2018
Abstract
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is a unique analytical technology that explores the spatial distribution of biomolecules (proteins, lipids, and metabolites) directly in situ, thus integrating molecular and morphological information. In particular, MALDI-MSI of tissue represents a new stimulating perspective in the field of proteomics and facilitates the detection of specific proteomic indicators that are directly correlated with disease alterations. Here we describe the key experimental steps for the MALDI-MSI analysis of both fresh-frozen and formalin-fixed paraffin-embedded tissue, in particular for clinically orientated studies. Furthermore, we highlight how this approach has been employed in the study of renal diseases and can provide proteomic signatures of the most common glomerular diseases.
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