Biomass combustion contributes to air pollution and is correlated to adverse health effects. This study aims to investigate the possible diverse cytoxicological properties of particles collected during the combustion of different biomass sources. The particles (PM10) derived from the combustion of pellet, charcoal and wood were collected and characterized for PAH and metal content. Human alveolar A549 cells were exposed for 24 h to 5ug/cm2 PM. Cell viability (Alamar Blue assay and Hoechst/PI staining), inflammatory response (IL-6 and IL-8), antioxidant activity (HO-1 expression), xenobiotic metabolism activation (CYP1A1/1B1) and DNA damage (yH2AX, phospho-ATM/ATR) were evaluated. The results revealed different biological responses after exposure to the different PMs, suggesting a possible correlation between the particles’ chemical properties and their toxicological profile. Pellet-derived PM affected cell viability, inducing necrosis, while charcoal and wood PMs mainly induced apoptosis. Only pellet-derived PM activated inflammatory pathways, through IL-6 and IL-8 synthesis. All the particles caused a significant increase of HO-1, confirming oxidative stress-related responses, and the activation of the cytochrome P450 enzymes. Finally, particles caused an increase in the level of DNA strand breaks. These data demonstrate that combustion particles deriving from different biomasses may activate different toxicological pathways, pointing out the importance of the biomass type and quality in the strategies to prevent respiratory diseases

Marchetti, S., Longhin, E., Bengalli, R., Buonanno, G., Colombo, A., Mantecca, P., et al. (2017). Comparative biological effects induced in A549 cells by combustion-derived particles from different biomass sources. In 53rd Congress of the European Societies of Toxicology (EUROTOX) (pp.S265-S265). Elsevier [10.1016/j.toxlet.2017.07.938].

Comparative biological effects induced in A549 cells by combustion-derived particles from different biomass sources

MARCHETTI, SARA
Primo
;
LONGHIN, ELEONORA MARTA
Secondo
;
BENGALLI, ROSSELLA DANIELA;COLOMBO, ANITA EMILIA;MANTECCA, PARIDE
Penultimo
;
CAMATINI, MARINA CARLA
Ultimo
2017

Abstract

Biomass combustion contributes to air pollution and is correlated to adverse health effects. This study aims to investigate the possible diverse cytoxicological properties of particles collected during the combustion of different biomass sources. The particles (PM10) derived from the combustion of pellet, charcoal and wood were collected and characterized for PAH and metal content. Human alveolar A549 cells were exposed for 24 h to 5ug/cm2 PM. Cell viability (Alamar Blue assay and Hoechst/PI staining), inflammatory response (IL-6 and IL-8), antioxidant activity (HO-1 expression), xenobiotic metabolism activation (CYP1A1/1B1) and DNA damage (yH2AX, phospho-ATM/ATR) were evaluated. The results revealed different biological responses after exposure to the different PMs, suggesting a possible correlation between the particles’ chemical properties and their toxicological profile. Pellet-derived PM affected cell viability, inducing necrosis, while charcoal and wood PMs mainly induced apoptosis. Only pellet-derived PM activated inflammatory pathways, through IL-6 and IL-8 synthesis. All the particles caused a significant increase of HO-1, confirming oxidative stress-related responses, and the activation of the cytochrome P450 enzymes. Finally, particles caused an increase in the level of DNA strand breaks. These data demonstrate that combustion particles deriving from different biomasses may activate different toxicological pathways, pointing out the importance of the biomass type and quality in the strategies to prevent respiratory diseases
No
abstract + poster
biomass, air pollution, in vitro lung cells, toxicity
English
53rd Congress of the European Societies of Toxicology (EUROTOX)
Marchetti, S., Longhin, E., Bengalli, R., Buonanno, G., Colombo, A., Mantecca, P., et al. (2017). Comparative biological effects induced in A549 cells by combustion-derived particles from different biomass sources. In 53rd Congress of the European Societies of Toxicology (EUROTOX) (pp.S265-S265). Elsevier [10.1016/j.toxlet.2017.07.938].
Marchetti, S; Longhin, E; Bengalli, R; Buonanno, G; Colombo, A; Mantecca, P; Camatini, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/170910
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