We studied the secretion of recombinant human insulin-like growth factor 1 (rhIGF-1) from transformed yeast cells. The hIGF-1 gene was fused to the mating factor α prepro-leader sequence under the control of the constitutive ACT1 promoter. We found that the inactivation of the GAS1 gene in the host strain led to a supersecretory phenotype yielding a considerable increase, from 8 to 55 mg/liter, in rhIGF-1 production.

Vai, M., Brambilla, L., Orlandi, I., Rota, N., Ranzi, B., Alberghina, L., et al. (2000). Improved secretion of native human IGF-1 from gas1 Saccharomyces cerevisiae cells. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 66, 5477-5479.

Improved secretion of native human IGF-1 from gas1 Saccharomyces cerevisiae cells

VAI, MARINA;BRAMBILLA, LUCA GIUSEPPE;ORLANDI, IVAN;ALBERGHINA, LILIA;PORRO, DANILO
2000

Abstract

We studied the secretion of recombinant human insulin-like growth factor 1 (rhIGF-1) from transformed yeast cells. The hIGF-1 gene was fused to the mating factor α prepro-leader sequence under the control of the constitutive ACT1 promoter. We found that the inactivation of the GAS1 gene in the host strain led to a supersecretory phenotype yielding a considerable increase, from 8 to 55 mg/liter, in rhIGF-1 production.
Articolo in rivista - Articolo scientifico
saccharomyces cerevisiae; IGF-1; GAS1; secretion
English
5477
5479
Vai, M., Brambilla, L., Orlandi, I., Rota, N., Ranzi, B., Alberghina, L., et al. (2000). Improved secretion of native human IGF-1 from gas1 Saccharomyces cerevisiae cells. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 66, 5477-5479.
Vai, M; Brambilla, L; Orlandi, I; Rota, N; Ranzi, B; Alberghina, L; Porro, D
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/16481
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