Samples of PM(2.5) were gravimetrically collected during the winter 2005/2006 in the urban area of Milan (North Italy). Samples were chemically characterized and the particles were detached from filters to determine their cytotoxic effects on the A549 cell line. Based on the potential toxicological relevance of its components, Milan winter PM(2.5) contained high concentrations of pro-oxidant transition metals and PAHs, while re-suspended particles showed a relatively high frequency of dimensional classes ranging from 40 nm to 300 nm. A549 cells exposed to particle suspensions showed a concentration-dependent decrease in viability, starting from 10 microg/cm(2). Phagocytosis of particles by A549 cells and particle aggregates were morphologically characterized and seemed to depend on both particle concentration and exposure time, with the majority of particles being engulfed in membrane-bound vacuoles after 24h of exposure. The ability of ultrafine particles to penetrate and spread throughout the cells was also verified. Cell membrane lysis and mitochondrial ultrastructural disruption appeared to be the main modifications induced by PM(2.5) on A549 cells. Concomitantly to the adverse effects observed in terms of cell mortality and ultrastructural lesions, a significant intracellular production of reactive oxygen species (ROS) was observed, suggesting that the cytotoxicity, exerted by the winter PM(2.5) in Milan, derived also from its oxidative potential, probably associated with particle-adsorbed metals and PAHs

Gualtieri, M., Mantecca, P., Corvaja, V., Longhin, E., Perrone, M., Bolzacchini, E., et al. (2009). Winter fine particulate matter from Milan induces morphological and functional alterations in human pulmonary epithelial cells (A549). TOXICOLOGY LETTERS, 188(1), 52-62 [10.1016/j.toxlet.2009.03.003].

Winter fine particulate matter from Milan induces morphological and functional alterations in human pulmonary epithelial cells (A549)

GUALTIERI, MAURIZIO;MANTECCA, PARIDE;CORVAJA, VIVIANA GIULIA INES;LONGHIN, ELEONORA MARTA;PERRONE, MARIA GRAZIA;BOLZACCHINI, EZIO;CAMATINI, MARINA CARLA
2009

Abstract

Samples of PM(2.5) were gravimetrically collected during the winter 2005/2006 in the urban area of Milan (North Italy). Samples were chemically characterized and the particles were detached from filters to determine their cytotoxic effects on the A549 cell line. Based on the potential toxicological relevance of its components, Milan winter PM(2.5) contained high concentrations of pro-oxidant transition metals and PAHs, while re-suspended particles showed a relatively high frequency of dimensional classes ranging from 40 nm to 300 nm. A549 cells exposed to particle suspensions showed a concentration-dependent decrease in viability, starting from 10 microg/cm(2). Phagocytosis of particles by A549 cells and particle aggregates were morphologically characterized and seemed to depend on both particle concentration and exposure time, with the majority of particles being engulfed in membrane-bound vacuoles after 24h of exposure. The ability of ultrafine particles to penetrate and spread throughout the cells was also verified. Cell membrane lysis and mitochondrial ultrastructural disruption appeared to be the main modifications induced by PM(2.5) on A549 cells. Concomitantly to the adverse effects observed in terms of cell mortality and ultrastructural lesions, a significant intracellular production of reactive oxygen species (ROS) was observed, suggesting that the cytotoxicity, exerted by the winter PM(2.5) in Milan, derived also from its oxidative potential, probably associated with particle-adsorbed metals and PAHs
Articolo in rivista - Articolo scientifico
Reactive Oxygen Species; Phagocytosis; Epithelial Cells; Time Factors; Seasons; Oxidative Stress; Particulate Matter; Lung; Italy; Dose-Response Relationship, Drug; Cell Shape; Phagosomes; Humans; Mitochondria; Cell Line, Tumor; Cell Survival; Particle Size; Polycyclic Hydrocarbons, Aromatic; Metals
English
52
62
11
Gualtieri, M., Mantecca, P., Corvaja, V., Longhin, E., Perrone, M., Bolzacchini, E., et al. (2009). Winter fine particulate matter from Milan induces morphological and functional alterations in human pulmonary epithelial cells (A549). TOXICOLOGY LETTERS, 188(1), 52-62 [10.1016/j.toxlet.2009.03.003].
Gualtieri, M; Mantecca, P; Corvaja, V; Longhin, E; Perrone, M; Bolzacchini, E; Camatini, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/15228
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