The yeast Zygosaccharomyces bailii, known to have peculiar resistance to several environmental constraints, is very little known with respect to its genetics and life cycle. In addition to molecular and biochemical studies, cytofluorimetric and morphological analyses can also add information necessary to shed light on its interesting features. In the present study, the DNA and protein content as well as the cellular morphology of Z. bailii populations growing in minimal medium supplemented with different carbon sources and with the addition of different organic acids were investigated. The results show the occurrence of a multibudded phenotype and of a low, but significant percentage of binucleate cells occurring in the early-stationary phase. These traits appear to be different in comparison with the better-known laboratory yeast Saccharomyces cerevisiae. Experiments and speculations about these features and possible implications with Z. bailii main characteristics are discussed.

Dato, L., Sauer, M., Passolunghi, S., Porro, D., & Branduardi, P. (2008). Investigating the multibudded and bi-nucleate phenotype of the yeast Zygosaccharomyces bailii growing on minimal medium. FEMS YEAST RESEARCH, 8(6), 906-915 [10.1111/j.1567-1364.2008.00417.x].

Investigating the multibudded and bi-nucleate phenotype of the yeast Zygosaccharomyces bailii growing on minimal medium

DATO, LAURA;PASSOLUNGHI, SIMONE;PORRO, DANILO;BRANDUARDI, PAOLA
2008

Abstract

The yeast Zygosaccharomyces bailii, known to have peculiar resistance to several environmental constraints, is very little known with respect to its genetics and life cycle. In addition to molecular and biochemical studies, cytofluorimetric and morphological analyses can also add information necessary to shed light on its interesting features. In the present study, the DNA and protein content as well as the cellular morphology of Z. bailii populations growing in minimal medium supplemented with different carbon sources and with the addition of different organic acids were investigated. The results show the occurrence of a multibudded phenotype and of a low, but significant percentage of binucleate cells occurring in the early-stationary phase. These traits appear to be different in comparison with the better-known laboratory yeast Saccharomyces cerevisiae. Experiments and speculations about these features and possible implications with Z. bailii main characteristics are discussed.
Si
Articolo in rivista - Articolo scientifico
Scientifica
Zygosaccharomyces bailii; flow cytometry, multibudded cells,binucleate cells
English
906
915
10
Dato, L., Sauer, M., Passolunghi, S., Porro, D., & Branduardi, P. (2008). Investigating the multibudded and bi-nucleate phenotype of the yeast Zygosaccharomyces bailii growing on minimal medium. FEMS YEAST RESEARCH, 8(6), 906-915 [10.1111/j.1567-1364.2008.00417.x].
Dato, L; Sauer, M; Passolunghi, S; Porro, D; Branduardi, P
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/14662
Citazioni
  • Scopus 5
  • ???jsp.display-item.citation.isi??? 6
Social impact