Purpose: Chronic tendinopathy is a degenerative process causing pain and disability. Current treatments include biophysical therapies, such as pulsed electromagnetic fields (PEMF). The aim of this study was to compare, for the first time, the functional in vitro response of human tendon cells to different dosages of PEMF, varying in field intensity and duration and number of exposures. Methods: Tendon cells, isolated from human semitendinosus and gracilis tendons (hTCs; n = 6), were exposed to different PEMF treatments (1.5 or 3 mT for 8 or 12 h, single or repeated treatments). Scleraxis (SCX), COL1A1, COL3A1 and vascular endothelial growth factor-A (VEGF-A) expression and cytokine production were assessed. Results: None of the different dosages provoked apoptotic events. Proliferation of hTCs was enhanced by all treatments, whereas only 3 mT-PEMF treatment increased cell viability. However, the single 1.5 mT-PEMF treatment elicited the highest up-regulation of SCX, VEGF-A and COL1A1 expression, and it significantly reduced COL3A1 expression with respect to untreated cells. The treated hTCs showed a significantly higher release of IL-1β, IL-6, IL-10 and TGF-β. Interestingly, the repeated 1.5 mT-PEMF significantly further increased IL-10 production. Conclusions: 1.5 mT-PEMF treatment was able to give the best results in in vitro healthy human tendon cell culture. Although the clinical relevance is not direct, this investigation should be considered an attempt to clarify the effect of different PEMF protocols on tendon cells, in particular focusing on the potential applicability of this cell source for regenerative medicine purpose, both in surgical and in conservative treatment for tendon disorders.

De Girolamo, L., Vigano', M., Galliera, E., Stanco, D., Setti, S., Marazzi, M., et al. (2015). In vitro functional response of human tendon cells to different dosages of low-frequency pulsed electromagnetic field. KNEE SURGERY, SPORTS TRAUMATOLOGY, ARTHROSCOPY, 23(11), 3443-3453 [10.1007/s00167-014-3143-x].

In vitro functional response of human tendon cells to different dosages of low-frequency pulsed electromagnetic field

VIGANO', MARCO
Secondo
;
2015

Abstract

Purpose: Chronic tendinopathy is a degenerative process causing pain and disability. Current treatments include biophysical therapies, such as pulsed electromagnetic fields (PEMF). The aim of this study was to compare, for the first time, the functional in vitro response of human tendon cells to different dosages of PEMF, varying in field intensity and duration and number of exposures. Methods: Tendon cells, isolated from human semitendinosus and gracilis tendons (hTCs; n = 6), were exposed to different PEMF treatments (1.5 or 3 mT for 8 or 12 h, single or repeated treatments). Scleraxis (SCX), COL1A1, COL3A1 and vascular endothelial growth factor-A (VEGF-A) expression and cytokine production were assessed. Results: None of the different dosages provoked apoptotic events. Proliferation of hTCs was enhanced by all treatments, whereas only 3 mT-PEMF treatment increased cell viability. However, the single 1.5 mT-PEMF treatment elicited the highest up-regulation of SCX, VEGF-A and COL1A1 expression, and it significantly reduced COL3A1 expression with respect to untreated cells. The treated hTCs showed a significantly higher release of IL-1β, IL-6, IL-10 and TGF-β. Interestingly, the repeated 1.5 mT-PEMF significantly further increased IL-10 production. Conclusions: 1.5 mT-PEMF treatment was able to give the best results in in vitro healthy human tendon cell culture. Although the clinical relevance is not direct, this investigation should be considered an attempt to clarify the effect of different PEMF protocols on tendon cells, in particular focusing on the potential applicability of this cell source for regenerative medicine purpose, both in surgical and in conservative treatment for tendon disorders.
Articolo in rivista - Articolo scientifico
Anti-inflammatory action; Human tendon cells; PEMF dosage; Pulsed electromagnetic field; Tendon markers; Adult; Basic Helix-Loop-Helix Transcription Factors; Cell Survival; Cells, Cultured; Collagen Type I; Collagen Type III; Humans; Interleukins; Tendons; Transforming Growth Factor beta; Up-Regulation; Vascular Endothelial Growth Factor A; Electromagnetic Fields; Surgery; Orthopedics and Sports Medicine
English
3443
3453
11
De Girolamo, L., Vigano', M., Galliera, E., Stanco, D., Setti, S., Marazzi, M., et al. (2015). In vitro functional response of human tendon cells to different dosages of low-frequency pulsed electromagnetic field. KNEE SURGERY, SPORTS TRAUMATOLOGY, ARTHROSCOPY, 23(11), 3443-3453 [10.1007/s00167-014-3143-x].
De Girolamo, L; Vigano', M; Galliera, E; Stanco, D; Setti, S; Marazzi, M; Thiebat, G; Corsi Romanelli, M; Sansone, V
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/143528
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