Background: The detection of anti-proteinase 3 (PR3) and anti-myeloperoxidase (MPO) autoantibodies represents a serological hallmark in the diagnosis of small vessel vasculitis such as granulomatosis with polyangiitis (GPA) and microscopic polyangiitis (MPA). We evaluated novel chemiluminescence assays (CIAs) for PR3- and MPO-ANCA detection and investigated their utility for disease activity monitoring. Methods: Sera collected from GPA (n = 41) and MPA (n = 30) patients were tested by QUANTA Lite® PR-3 and MPO ELISAs (INOVA Diagnostics) and by the QUANTA Flash™ PR3 and MPO CIAs (INOVA). Precision and linearity were analyzed following reference guidelines. The recently launched reference sera for PR3-and MPO-ANCA (Centers of Disease Control and prevention, CDC) were used to establish international units for the new assays. Disease activity was determined using the Birmingham Vasculitis Activity Score. Results: The international standards for PR3-and MPO-ANCA yielded results of 403. CU and 332. CU in the novel CIAs, respectively. The linearity analysis showed linear regression values > 0.97 with slopes between 0.96 and 1.04. Total variation obtained from the precision study showed CV% of ≤ 7.4 for PR3-ANCA and ≤ 12.8 for MPO-ANCA. Good agreement (Spearman rho ≥ 0.89) was observed between CIA and ELISA. PR3-ANCA determined by CIA, but not by ELISA, was correlated with disease activity. No correlation was found for MPO-ANCA. Conclusion: The novel PR3- and MPO-ANCA CIAs show good precision, linearity and correlation to ELISA. In addition, PR3-ANCA by CIA show correlation with disease activity. © 2012 Elsevier B.V.

Mahler, M., Radice, A., Yang, W., Bentow, C., Seaman, A., Bianchi, L., et al. (2012). Development and performance evaluation of novel chemiluminescence assays for detection of anti-PR3 and anti-MPO antibodies. CLINICA CHIMICA ACTA, 413(7-8), 719-726 [10.1016/j.cca.2012.01.004].

Development and performance evaluation of novel chemiluminescence assays for detection of anti-PR3 and anti-MPO antibodies

SINICO, RENATO ALBERTO
Ultimo
2012

Abstract

Background: The detection of anti-proteinase 3 (PR3) and anti-myeloperoxidase (MPO) autoantibodies represents a serological hallmark in the diagnosis of small vessel vasculitis such as granulomatosis with polyangiitis (GPA) and microscopic polyangiitis (MPA). We evaluated novel chemiluminescence assays (CIAs) for PR3- and MPO-ANCA detection and investigated their utility for disease activity monitoring. Methods: Sera collected from GPA (n = 41) and MPA (n = 30) patients were tested by QUANTA Lite® PR-3 and MPO ELISAs (INOVA Diagnostics) and by the QUANTA Flash™ PR3 and MPO CIAs (INOVA). Precision and linearity were analyzed following reference guidelines. The recently launched reference sera for PR3-and MPO-ANCA (Centers of Disease Control and prevention, CDC) were used to establish international units for the new assays. Disease activity was determined using the Birmingham Vasculitis Activity Score. Results: The international standards for PR3-and MPO-ANCA yielded results of 403. CU and 332. CU in the novel CIAs, respectively. The linearity analysis showed linear regression values > 0.97 with slopes between 0.96 and 1.04. Total variation obtained from the precision study showed CV% of ≤ 7.4 for PR3-ANCA and ≤ 12.8 for MPO-ANCA. Good agreement (Spearman rho ≥ 0.89) was observed between CIA and ELISA. PR3-ANCA determined by CIA, but not by ELISA, was correlated with disease activity. No correlation was found for MPO-ANCA. Conclusion: The novel PR3- and MPO-ANCA CIAs show good precision, linearity and correlation to ELISA. In addition, PR3-ANCA by CIA show correlation with disease activity. © 2012 Elsevier B.V.
Articolo in rivista - Articolo scientifico
ELISA; GPA; Microscopic polyangiitis; MPA; MPO-ANCA; PR3-ANCA; Wegener's granulomatosis; Autoantibodies; Enzyme-Linked Immunosorbent Assay; Humans; Luminescence; Myeloblastin; Peroxidase; Reference Values; Reproducibility of Results; Biochemistry; Clinical Biochemistry; Biochemistry (medical)
English
719
726
8
Mahler, M., Radice, A., Yang, W., Bentow, C., Seaman, A., Bianchi, L., et al. (2012). Development and performance evaluation of novel chemiluminescence assays for detection of anti-PR3 and anti-MPO antibodies. CLINICA CHIMICA ACTA, 413(7-8), 719-726 [10.1016/j.cca.2012.01.004].
Mahler, M; Radice, A; Yang, W; Bentow, C; Seaman, A; Bianchi, L; Sinico, R
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/139253
Citazioni
  • Scopus 58
  • ???jsp.display-item.citation.isi??? 57
Social impact