Yeasts do not possess an endogenous biochemical pathway for the synthesis of vitamin C. However, incubated with L-galactose, L-galactono-1,4-lactone, or L-gulono-1,4-lactone intermediates from the plant or animal pathway leading to L-ascorbic acid, Saccharomyces cerevisiae and Zygosaccharomyces bailii cells accumulate the vitamin intracellularly. Overexpression of the S. cerevisiae enzymes D-arabinose dehydrogenase and D-arabinono-1,4-lactone oxidase enhances this ability significantly. In fact, the respective recombinant yeast strains even gain the capability to accumulate the vitamin in the culture medium. An even better result is obtainable by expression of the plant enzyme L-galactose dehydrogenase from Arabidopsis thaliana. Budding yeast cells overexpressing the endogenous D-arabinono-1,4-lactone oxidase as well as L-galactose dehydrogenase are capable of producing about 100 mg of L-ascorbic acid liter<sup>-1</sup>, converting 40% (wt/vol) of the starting compound L-galactose.

Sauer, M., Branduardi, P., Valli, M., Porro, D. (2004). Production of L-ascorbic acid by metabolically engineered Saccharomyces cerevisiae and Zygosaccharomyces bailii. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(10), 6086-6091 [10.1128/AEM.70.10.6086-6091.2004].

Production of L-ascorbic acid by metabolically engineered Saccharomyces cerevisiae and Zygosaccharomyces bailii

BRANDUARDI, PAOLA;PORRO, DANILO
2004

Abstract

Yeasts do not possess an endogenous biochemical pathway for the synthesis of vitamin C. However, incubated with L-galactose, L-galactono-1,4-lactone, or L-gulono-1,4-lactone intermediates from the plant or animal pathway leading to L-ascorbic acid, Saccharomyces cerevisiae and Zygosaccharomyces bailii cells accumulate the vitamin intracellularly. Overexpression of the S. cerevisiae enzymes D-arabinose dehydrogenase and D-arabinono-1,4-lactone oxidase enhances this ability significantly. In fact, the respective recombinant yeast strains even gain the capability to accumulate the vitamin in the culture medium. An even better result is obtainable by expression of the plant enzyme L-galactose dehydrogenase from Arabidopsis thaliana. Budding yeast cells overexpressing the endogenous D-arabinono-1,4-lactone oxidase as well as L-galactose dehydrogenase are capable of producing about 100 mg of L-ascorbic acid liter-1, converting 40% (wt/vol) of the starting compound L-galactose.
Articolo in rivista - Articolo scientifico
Saccharomyces cerevisiae, L-ascorbic acid, metabolic engineering
English
2004
70
10
6086
6091
none
Sauer, M., Branduardi, P., Valli, M., Porro, D. (2004). Production of L-ascorbic acid by metabolically engineered Saccharomyces cerevisiae and Zygosaccharomyces bailii. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(10), 6086-6091 [10.1128/AEM.70.10.6086-6091.2004].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/13843
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