Among plants, several groups have toxicities that are dangerous for humans. Plant exposures are indeed one of the most frequent cases reported to poison control centers. A rapid and easy screening test is required for accurate medical treatment. However, it is usually based on the morphological analysis of ingested plant portions that is expensive in terms of time and resources requiring experience in systematic botany which could also be biased by the absence of clear diagnostic traits in stomach contents. In this study, we designed specific primer pairs for the amplification of a sequence-characterized amplified region within standard barcode regions (i.e., rbcL and trnH–psbA) to detect toxic plants belonging to the genera Atropa and Colchicum. Allied edible species were also included in our data-set to exclude the cross-amplification with the toxic ones when the detection system is applied. Using real-time polymerase chain reaction, we tested the specificity of the selected primer pairs in order to develop a fast and reliable tool to be used in poison centers.

Federici, S., Fontana, D., Galimberti, A., Bruni, I., DE MATTIA, F., Cortis, P., et al. (2015). A rapid diagnostic approach to identify poisonous plants using DNA barcoding data. PLANT BIOSYSTEMS, 149(3), 537-545 [10.1080/11263504.2014.941031].

A rapid diagnostic approach to identify poisonous plants using DNA barcoding data

FEDERICI, SILVIA
;
GALIMBERTI, ANDREA;BRUNI, ILARIA;DE MATTIA, FABRIZIO;LABRA, MASSIMO
Ultimo
2015

Abstract

Among plants, several groups have toxicities that are dangerous for humans. Plant exposures are indeed one of the most frequent cases reported to poison control centers. A rapid and easy screening test is required for accurate medical treatment. However, it is usually based on the morphological analysis of ingested plant portions that is expensive in terms of time and resources requiring experience in systematic botany which could also be biased by the absence of clear diagnostic traits in stomach contents. In this study, we designed specific primer pairs for the amplification of a sequence-characterized amplified region within standard barcode regions (i.e., rbcL and trnH–psbA) to detect toxic plants belonging to the genera Atropa and Colchicum. Allied edible species were also included in our data-set to exclude the cross-amplification with the toxic ones when the detection system is applied. Using real-time polymerase chain reaction, we tested the specificity of the selected primer pairs in order to develop a fast and reliable tool to be used in poison centers.
Articolo in rivista - Articolo scientifico
Atropa belladonna; Colchicum spp; poison centers; sequence-characterized amplified region;
Atropa belladonna; Colchicum spp; poison centers; sequence-characterized amplified region; Plant Science; Ecology, Evolution, Behavior and Systematics
English
2015
149
3
537
545
none
Federici, S., Fontana, D., Galimberti, A., Bruni, I., DE MATTIA, F., Cortis, P., et al. (2015). A rapid diagnostic approach to identify poisonous plants using DNA barcoding data. PLANT BIOSYSTEMS, 149(3), 537-545 [10.1080/11263504.2014.941031].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/121931
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