A large number of hDAF transgenic pigs to be used for xenotransplantation research were generated by using sperm-mediated gene transfer (SMGT). The efficiency of transgenesis obtained with SMGT was much greater than with any other method. In the experiments reported, up to 80% of pigs had the transgene integrated into the genome. Most of the pigs carrying the hDAF gene transcribed it in a stable manner (64%). The great majority of pigs that transcribed the gene expressed the protein (83%). The hDAF gene was transmitted to progeny. Expression was stable and found in caveolae as it is in human cells. The expressed gene was functional based on in vitro experiments performed on peripheral blood mononuclear cells. These results show that our SMGT approach to transgenesis provides an efficient procedure for studies involving large animal models

Lavitrano, M., Bacci, M., Forni, M., Lazzereschi, D., Di Stefano, C., Fioretti, D., et al. (2002). Efficient production by sperm-mediated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 99, 14230-14235 [10.1073/pnas.222550299].

Efficient production by sperm-mediated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation

LAVITRANO, MARIALUISA;GIOVANNONI, ROBERTO;
2002-10-22

Abstract

A large number of hDAF transgenic pigs to be used for xenotransplantation research were generated by using sperm-mediated gene transfer (SMGT). The efficiency of transgenesis obtained with SMGT was much greater than with any other method. In the experiments reported, up to 80% of pigs had the transgene integrated into the genome. Most of the pigs carrying the hDAF gene transcribed it in a stable manner (64%). The great majority of pigs that transcribed the gene expressed the protein (83%). The hDAF gene was transmitted to progeny. Expression was stable and found in caveolae as it is in human cells. The expressed gene was functional based on in vitro experiments performed on peripheral blood mononuclear cells. These results show that our SMGT approach to transgenesis provides an efficient procedure for studies involving large animal models
Articolo in rivista - Articolo scientifico
SMGT, hyperacute rejection, swine
English
Lavitrano, M., Bacci, M., Forni, M., Lazzereschi, D., Di Stefano, C., Fioretti, D., et al. (2002). Efficient production by sperm-mediated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 99, 14230-14235 [10.1073/pnas.222550299].
Lavitrano, M; Bacci, M; Forni, M; Lazzereschi, D; Di Stefano, C; Fioretti, D; Giancotti, P; Marfé, G; Pucci, L; Renzi, L; Wang, H; Stoppacciaro, A; Stassi, G; Sargiacomo, M; Sinibaldi, P; Turchi, V; Giovannoni, R; Della Casa, G; Seren, E; Rossi, G
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/11084
Citazioni
  • Scopus 150
  • ???jsp.display-item.citation.isi??? 142
Social impact