Overexpression of hybrid Virus-Like-Particles (VLP) was found to dramatically affect cell kinetics and morphology with a significant increase in doubling times, induction times and major alterations in cell volume distributions. Optimal expression (ca. 10 mg/l) was obtained with a two-step fermentation strategy (raffinose → galactose) coupled to GAL4 overexpression, resulting in partial cell lysis and VLP release.

Cavadini, P., Popolo, L., Vai, M., Varona, R., Garofano, L., & Vanoni, M. (1994). In Saccharomyces cerevisiae overexpression of hybrid Virus-Like-Particles correlates with altered cell volume distributions. BIOTECHNOLOGY LETTERS, 16, 1131-1134 [10.1007/BF01020838].

In Saccharomyces cerevisiae overexpression of hybrid Virus-Like-Particles correlates with altered cell volume distributions

VAI, MARINA;VANONI, MARCO ERCOLE
1994

Abstract

Overexpression of hybrid Virus-Like-Particles (VLP) was found to dramatically affect cell kinetics and morphology with a significant increase in doubling times, induction times and major alterations in cell volume distributions. Optimal expression (ca. 10 mg/l) was obtained with a two-step fermentation strategy (raffinose → galactose) coupled to GAL4 overexpression, resulting in partial cell lysis and VLP release.
No
Articolo in rivista - Articolo scientifico
Scientifica
Virus-Like-Particles; two-step fermentation; GAL4 overexpression
English
1131
1134
4
Cavadini, P., Popolo, L., Vai, M., Varona, R., Garofano, L., & Vanoni, M. (1994). In Saccharomyces cerevisiae overexpression of hybrid Virus-Like-Particles correlates with altered cell volume distributions. BIOTECHNOLOGY LETTERS, 16, 1131-1134 [10.1007/BF01020838].
Cavadini, P; Popolo, L; Vai, M; Varona, R; Garofano, L; Vanoni, M
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/99047
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