Tunicamycin, an inhibitor of one of the earliest steps in the synthesis of N-linked oligosaccharides, prevents bud formation and growth in Saccharomyces cerevisiae cells that are either growing exponentially or recovering from different cell cycle arrests at start. Analysis of tunicamycin-treated cells by flow microfluorometry clearly shows that cells have a postsynthetic DNA content, but there is no evidence of an increase in binucleate cells. Therefore tunicamycin affects bud emergence and initiation of DNA synthesis, two events correlated under physiological conditions, in different ways. A bulk glycoprotein synthesis is shown to be required for bud emergence and localized chitin deposition, probably to sustain directional secretory vesicle transport, which allows polar growth of the bud. No evidence for a glycoprotein requirement for entrance into the S phase is obtained from the present experiments.

Vai, M., Popolo, L., & Alberghina, L. (1987). Effect of tunicamycin on cell cycle progression in budding yeast. EXPERIMENTAL CELL RESEARCH, 171, 448-459.

Effect of tunicamycin on cell cycle progression in budding yeast

VAI, MARINA
Primo
;
ALBERGHINA, LILIA
Ultimo
1987

Abstract

Tunicamycin, an inhibitor of one of the earliest steps in the synthesis of N-linked oligosaccharides, prevents bud formation and growth in Saccharomyces cerevisiae cells that are either growing exponentially or recovering from different cell cycle arrests at start. Analysis of tunicamycin-treated cells by flow microfluorometry clearly shows that cells have a postsynthetic DNA content, but there is no evidence of an increase in binucleate cells. Therefore tunicamycin affects bud emergence and initiation of DNA synthesis, two events correlated under physiological conditions, in different ways. A bulk glycoprotein synthesis is shown to be required for bud emergence and localized chitin deposition, probably to sustain directional secretory vesicle transport, which allows polar growth of the bud. No evidence for a glycoprotein requirement for entrance into the S phase is obtained from the present experiments.
No
Articolo in rivista - Articolo scientifico
Scientifica
Cell Cycle Progression; Saccharomyces cerevisiae; Tunicamycin
English
Vai, M., Popolo, L., & Alberghina, L. (1987). Effect of tunicamycin on cell cycle progression in budding yeast. EXPERIMENTAL CELL RESEARCH, 171, 448-459.
Vai, M; Popolo, L; Alberghina, L
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/99041
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