We investigated naturally occurring variation within the major (L1) and minor (L2) capsid proteins of oncogenic human papillomavirus (HPV) genotype 31 (HPV31) to determine the impact on capsid antigenicity. L1L2 pseudoviruses (PsVs) representing the three HPV31 variant lineages, variant lineages A, B, and C, exhibited comparable particle-to-infectivity ratios and morphologies. Lineage-specific L1L2 PsVs demonstrated subtle differences in susceptibility to neutralization by antibodies elicited following vaccination or preclinical L1 virus-like particle (VLP) immunization or by monoclonal antibodies; however, these differences were generally of a low magnitude. These data indicate that the diagnostic lineage-specific single nucleotide polymorphisms within the HPV31 capsid genes have a limited effect on L1 antibody-mediated neutralization and that the three HPV31 variant lineages belong to a single L1 serotype. These data contribute to our understanding of HPV L1 variant antigenicity.

Bissett, S., Godi, A., Fleury, M., Touze, A., Cocuzza, C., & Beddows, S. (2015). Naturally occurring capsid protein variants of human papillomavirus genotype 31 represent a single L1 serotype. JOURNAL OF VIROLOGY, 89(15), 7748-7757 [10.1128/JVI.00842-15].

Naturally occurring capsid protein variants of human papillomavirus genotype 31 represent a single L1 serotype

Cocuzza, C;
2015

Abstract

We investigated naturally occurring variation within the major (L1) and minor (L2) capsid proteins of oncogenic human papillomavirus (HPV) genotype 31 (HPV31) to determine the impact on capsid antigenicity. L1L2 pseudoviruses (PsVs) representing the three HPV31 variant lineages, variant lineages A, B, and C, exhibited comparable particle-to-infectivity ratios and morphologies. Lineage-specific L1L2 PsVs demonstrated subtle differences in susceptibility to neutralization by antibodies elicited following vaccination or preclinical L1 virus-like particle (VLP) immunization or by monoclonal antibodies; however, these differences were generally of a low magnitude. These data indicate that the diagnostic lineage-specific single nucleotide polymorphisms within the HPV31 capsid genes have a limited effect on L1 antibody-mediated neutralization and that the three HPV31 variant lineages belong to a single L1 serotype. These data contribute to our understanding of HPV L1 variant antigenicity.
Articolo in rivista - Articolo scientifico
Human Papillomavirus, Capsid Protein Variants, immunity
English
7748
7757
10
Bissett, S., Godi, A., Fleury, M., Touze, A., Cocuzza, C., & Beddows, S. (2015). Naturally occurring capsid protein variants of human papillomavirus genotype 31 represent a single L1 serotype. JOURNAL OF VIROLOGY, 89(15), 7748-7757 [10.1128/JVI.00842-15].
Bissett, S; Godi, A; Fleury, M; Touze, A; Cocuzza, C; Beddows, S
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/98280
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